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BioVector® NTCC® pYL69
PhCMV-driven expression of Gln125,232-lysostaphin N-terminally fused to human IgG-Fc with linker sequence of (SGGGG)3S secreted by a humanized form of Gaussia luciferase signal peptide (PhCMV-hGLuc-hFc-Lys-pA). hGLuc was first created by annealing OYL76 (5’-CCCCCCAAGCTTGCCCACCATGGGAGTCAAAGTTCTGTTTGCCCTGATCTGCATCGCTGTGGCCGAGGCC-3’, HindIII underlined) with OYL77 (5’- TTGTCAGATCTAACCATGGCCGATATGGCCTCGGCCACAGC-3’). This product was then annealed with human IgG-Fc, which was PCR amplified from pHY79 using OYL108 (5’-ATATCGGCCATGGTTAGATCTGACAA-3’) and OYL109 (5’-TGATCCGCCGCCACCCGACCCACCTCCGCCCGAGCCTCCGCCACCAGATTATTTACCCGGAGAC-3’, linker sequence underlined), to create hGLuc-hFc. hGLuc-hFc-Lys was created by annealing hGLuc-hFc with Gln125,232-lysostaphin, a PCR amplification of pYL33 using OYL110 (5’-TCTGGTGGCGGAGGCTCGGGCGGAGGTGGGTCGGGTGGCGGCGGATCAGCTACTCACGAACACT-3’, linker sequence underlined) and OYL73 (5’-TGCCTAGTCTAGATTATTTGATAGTGCCCCACA-3’, XbaI underlined), and subsequently restricted with HindIII/XbaI and ligated into the corresponding sites (HindIII/XbaI) of pcDNA3.1(+).
Supplier来源:BioVector NTCC Inc.
Email: biovector@163.com
Website网址: http://www.biovector.net
PhCMV-driven expression of Gln125,232-lysostaphin N-terminally fused to human IgG-Fc with linker sequence of (SGGGG)3S secreted by a humanized form of Gaussia luciferase signal peptide (PhCMV-hGLuc-hFc-Lys-pA). hGLuc was first created by annealing OYL76 (5’-CCCCCCAAGCTTGCCCACCATGGGAGTCAAAGTTCTGTTTGCCCTGATCTGCATCGCTGTGGCCGAGGCC-3’, HindIII underlined) with OYL77 (5’- TTGTCAGATCTAACCATGGCCGATATGGCCTCGGCCACAGC-3’). This product was then annealed with human IgG-Fc, which was PCR amplified from pHY79 using OYL108 (5’-ATATCGGCCATGGTTAGATCTGACAA-3’) and OYL109 (5’-TGATCCGCCGCCACCCGACCCACCTCCGCCCGAGCCTCCGCCACCAGATTATTTACCCGGAGAC-3’, linker sequence underlined), to create hGLuc-hFc. hGLuc-hFc-Lys was created by annealing hGLuc-hFc with Gln125,232-lysostaphin, a PCR amplification of pYL33 using OYL110 (5’-TCTGGTGGCGGAGGCTCGGGCGGAGGTGGGTCGGGTGGCGGCGGATCAGCTACTCACGAACACT-3’, linker sequence underlined) and OYL73 (5’-TGCCTAGTCTAGATTATTTGATAGTGCCCCACA-3’, XbaI underlined), and subsequently restricted with HindIII/XbaI and ligated into the corresponding sites (HindIII/XbaI) of pcDNA3.1(+).
Supplier来源:BioVector NTCC Inc.
Email: biovector@163.com
Website网址: http://www.biovector.net
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