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BioVector® NTCC® pYL52
Constitutive Gln125,232-lysostaphin expression directed by mouse Igκ-chain leader sequence for secretion (PhCMV-mIgκ-Lys-pA). mIgκ-Lys was PCR amplified from pYL33 using OYL142 (5’-CCCCCCAAGCTTCCACCATGGAGACAGACACACTCCTGCTATGGGTACTGCTGCTCTGGGTTCCAGGTTCCACTGGTGACGCTACTCACGAACACTC-3’, HindIII underlined) and OYL73 (5’-TGCCTAGTCTAGATTATTTGATAGTGCCCCACA-3’, XbaI underlined), digested with HindIII/XbaI and cloned into pcDNA3.1(+) (HindIII/XbaI).
Supplier来源:BioVector NTCC Inc.
Email: biovector@163.com
Website网址: http://www.biovector.net
Constitutive Gln125,232-lysostaphin expression directed by mouse Igκ-chain leader sequence for secretion (PhCMV-mIgκ-Lys-pA). mIgκ-Lys was PCR amplified from pYL33 using OYL142 (5’-CCCCCCAAGCTTCCACCATGGAGACAGACACACTCCTGCTATGGGTACTGCTGCTCTGGGTTCCAGGTTCCACTGGTGACGCTACTCACGAACACTC-3’, HindIII underlined) and OYL73 (5’-TGCCTAGTCTAGATTATTTGATAGTGCCCCACA-3’, XbaI underlined), digested with HindIII/XbaI and cloned into pcDNA3.1(+) (HindIII/XbaI).
Supplier来源:BioVector NTCC Inc.
Email: biovector@163.com
Website网址: http://www.biovector.net
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