BioVector® pUV15-pHGFP plasmid vector结核分枝杆菌pH敏感性荧光表达质粒载体 BioVector NTCC质粒载体菌种细胞基因保藏中心

BioVector® pUV15-pHGFP plasmid vector结核分枝杆菌pH敏感性荧光表达质粒载体

encodes a pH-sensitive ratiometric GFP (pH-GFP), which allows for noninvasive measurements of intrabacterial pH in live cells
The pH-GFP insert in this plasmid contains a F220L mutation compared to the wild-type pHluorin. This difference does not affect function of the pH-GFP.
Vector typeMycobacterium tuberculosis expression vector
GROWTH IN BACTERIA
Bacterial Resistance(s)Hygromycin, 200 μg/mL
Growth Temperature37°C
Growth Strain(s)DH5alpha
Copy numberUnknown
GENE/INSERT
Gene/Insert namephGFP
Alt namepH-sensitive ratiometric GFP
SpeciesSynthetic
Insert Size (bp)700
Promotermycobacterial promoter Psmyc
CLONING INFORMATION
Cloning methodRestriction Enzyme
5′ cloning sitePacI (not destroyed)
3′ cloning siteEcoRV (not destroyed)
5′ sequencing primerpsmyc1 CGACCAGCACGGCATACATC
3′ sequencing primerpBluescript-KS

Supplier来源:BioVector NTCC Inc.
TEL电话:400-800-2947
Website网址: http://www.biovector.net