INA-6 human multiple myeloma cell line人多发性骨髓瘤细胞株 BioVector NTCC质粒载体菌种细胞基因保藏中心

INA-6 human multiple myeloma cell line人多发性骨髓瘤细胞株

Cell line:
INA-6
NTCC® No.:
NTCC-931732

Species:
human (Homo sapiens)
Cell type:
multiple myeloma

Origin:
established from the pleural effusion of an 80-year old patient with plasma cell leukemia in 1988. Cell line is IL-6 dependent and was described in the literature to carry an activating mutation of the N-RAS gene and del(1)(p22), del(7)(p13) and 14q+: t(11;14)(q13;q32) translocation

NTCC® Cell Culture Data:
Morphology:
round cells of different sizes, growing singly or in small clumps in suspension;


Medium:
80% RPMI 1640 + 20% FBS + 10 ng/ml IL-6
Subculture:
seed out 0.5-1.0 x 106 cells/well in a 24-well plate or at 0.5 x 10^6 cells/ml in a small culture flask; maintain at 0.5-1.5 x 10^6 cells/ml, split 1:2 to 1:4 every 2-3 days. After thawing growth may be delayed

Incubation:
at 37 °C with 5% CO2
Doubling time:
about 30-50 hours
Harvest:
maximal density of ca. 2 x 10^6 cells/ml
Storage:
frozen with 70% medium, 20% FBS, 10% DMSO

NTCC® Scientific Data:
Mycoplasma:
negative in PCR assay

Immunology:
CD3 -, CD10 -, CD13 -, CD19 -, CD20 -, CD34 -, CD37 -, CD38 -, cyCD79a -, CD80 -, CD138 +, HLA-DR -, sm/cyIgG -, sm/cyIgM -, smkappa -, cykappa +, sm/cylambda -;




Authenticity:
STR analysis according to the global standard ANSI/ATCC ASN-0002.1-2021 (2021) resulted in an authentic STR profile of the reference STR database.

Viruses:
PCR: EBV -, HBV -, HCV -, HIV-1 -, HIV-2 -, HTLV-1/2 -, MLV -

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