pMS2-1 and pMS2-2 plasmid vector质粒载体 BioVector NTCC质粒载体菌种细胞基因保藏中心

pMS2-1 and pMS2-2 plasmid vector质粒载体

pMS2-1 and pMS2-2. These two plasmids can be used to facilitate making the Hybrid RNA
construct (see below). Both are TETR vectors (the AMPR gene has been inactivated). DNA encoding
the RNA sequence of interest can be cloned into these plasmids, then moved into pIIIEx426RPR (see
below). pMS2-1 and pMS2-2 are based on BluescriptIIKS+ and do not contain any yeast genes or
promoters. They simply allow you to place the RNA sequence of interest next to two MS2 coat
protein recognition sites, then excise the relevant DNA fragment to be moved into pIIIEx426RPR.
pMS2-1 and pMS2-2 differ only in the relative positions of the MS2 sites and your sequence of
interest

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