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Yeast strain YBZ1 酵母菌株 BioVector NTCC质粒载体菌种细胞基因保藏中心

  • 价  格:¥49850
  • 货  号:Yeast strain YBZ1
  • 产  地:北京
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Yeast strain YBZ1 酵母菌株

Yeast strain YBZ1. A derivative of L40-coat carrying a tandem, head-to-tail dimer of MS2 coat
protein fused to a single LexA monomer. A nine-amino-acid linker (Gly-Ala-Pro-Gly-Ile-His-ProGly-Met) was introduced to enable flexibility in orienting the two MS2 segments. In addition, each
coat protein segment also carries a point mutation (N55K) that decreases the Kd of the RNA-protein
interaction. The genotype of YBZ1 is MATa, ura3-52, leu2-3, 112, his3-200, trp1-1, ade2, LYS2 ::
(LexAop)-HIS3, ura3 :: (lexA-op)-lacZ, LexA-MS2 coat (N55K). Hook, B., D. Bernstein, et al.
(2005). "RNA-protein interactions in the yeast three-hybrid system: affinity, sensitivity, and enhanced
library screening." RNA 11(2): 227-33

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