NTCC® 293T-CD3 cell line稳定表达细胞株293T-TCR:CD3 BioVector NTCC质粒载体菌种细胞基因保藏中心
- 价 格：¥989850
- 货 号：293T-CD3
- 产 地：北京
- BioVector NTCC典型培养物保藏中心
- 联系人：Dr.Xu, Biovector NTCC Inc.
电话：400-800-2947 工作QQ:1843439339 (微信同号)
邮件：Biovector@163.com
手机：18901268599
地址：北京
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NTCC® 293T-CD3 cell line稳定表达细胞株293T-TCR:CD3
Efficient cleavage and expression of TCR:CD3 complexes on 293T and 3T3 cells using 2A-linked vectors.(a) 293T cells were transiently transfected with plasmids carrying the individual or 2A-linked TCR:CD3 chains indicated. Cells were stained with anti-TCR-.PE, anti-CD3.APC or both and analyzed by flow cytometry. The percentage of live, TCR+CD3+ cells is shown. (b) Supernatant from retroviral producers expressing 2A peptide–linked TCR:CD3 chains was used to transduce 3T3 cells. Cells were analyzed as in a. (c) 3T3 cells transduced with separate or 2A-linked TCR:CD3 chains were surface biotinylated and lysed in digitonin. Untransduced cells were used as a control (negative). Lysates were immunoprecipitated with anti-TCR- and the eluted proteins resolved by SDS-PAGE. Membranes were probed with streptavidin or antibodies. *, a nonspecific 26–27 kDa band was observed in both the negative and transduced cells after western blot with anti-CD3.
Supplier来源:BioVector NTCC Inc.
TEL电话:400-800-2947
Website网址: http://www.biovector.net
Efficient cleavage and expression of TCR:CD3 complexes on 293T and 3T3 cells using 2A-linked vectors.(a) 293T cells were transiently transfected with plasmids carrying the individual or 2A-linked TCR:CD3 chains indicated. Cells were stained with anti-TCR-.PE, anti-CD3.APC or both and analyzed by flow cytometry. The percentage of live, TCR+CD3+ cells is shown. (b) Supernatant from retroviral producers expressing 2A peptide–linked TCR:CD3 chains was used to transduce 3T3 cells. Cells were analyzed as in a. (c) 3T3 cells transduced with separate or 2A-linked TCR:CD3 chains were surface biotinylated and lysed in digitonin. Untransduced cells were used as a control (negative). Lysates were immunoprecipitated with anti-TCR- and the eluted proteins resolved by SDS-PAGE. Membranes were probed with streptavidin or antibodies. *, a nonspecific 26–27 kDa band was observed in both the negative and transduced cells after western blot with anti-CD3.
Supplier来源:BioVector NTCC Inc.
TEL电话:400-800-2947
Website网址: http://www.biovector.net
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