pBMN FKBP(DD)-YFP plasmid vector逆病毒荧光表达质粒载体 BioVector NTCC质粒载体菌种细胞基因保藏中心

pBMN FKBP(DD)-YFP plasmid vector逆病毒荧光表达质粒载体

pBMN
(Search Vector Database)
Backbone manufacturerGarry Nolan
Backbone size w/o insert (bp)6943
Vector typeRetroviral
Selectable markersHcRed fluorescence behind IRES
GROWTH IN BACTERIA
Bacterial Resistance(s)Ampicillin, 100 μg/mL
Growth Temperature30°C
Growth Strain(s)Top10/P3
Growth instructionsCulture at 30C to prevent recombination of LTR and lost insert. Takes >20 hours to get good culture at 30C
Copy numberLow Copy
GENE/INSERT
Gene/Insert nameFKBP(DD)-YFP-HA
Alt nameYFP and FKBP12 (destable domain)
Alt nameYFP fused with FK506 binding protein 1A( destable domain)
SpeciesH. sapiens (human)
MutationF36V, L106P on FKBP12
GenBank IDNM_000801
Entrez GeneFKBP1A (a.k.a. FKBP-12, FKBP-1A, FKBP1, FKBP12, PKC12, PKCI2, PPIASE)
PromoterLTR
Tags / Fusion Proteins
YFP (C terminal on insert)
HA (C terminal on backbone)
CLONING INFORMATION
Cloning methodRestriction Enzyme
5′ cloning siteBamHI (not destroyed)
3′ cloning siteXhoI (not destroyed)
5′ sequencing primerpBMN specific

Supplier来源:BioVector NTCC Inc.
TEL电话:400-800-2947
Website网址: http://www.biovector.net