CHO-K1-GPR139 cell line细胞株 BioVector NTCC质粒载体菌种细胞基因保藏中心

CHO-K1-GPR139 cell line细胞株

Cell line name PathHunter CHO-K1 GPR139 beta-arrestin Accession NTCC®_KX32 Resource Identification Initiative To cite this cell line use: PathHunter CHO-K1 GPR139 beta-arrestin (NTCC®_KX32) Comments Characteristics: PathHunter beta-arrestin cell lines co-express a GPCR tagged with a beta-galactosidase fragment (ProLink=PK) and a beta-arrestin tagged with another fragment (Enzyme Acceptor=EA). Activation of the GPCR-PK induces beta-arrestin-EA recruitment and complementation of the PK and EA enzyme fragments thus resulting into a catalytically active enzyme that generate a chemiluminescent signal. Transfected with: HGNC; 19995; GPR139. Transfected with: HGNC; 712; ARRB2. Derived from sampling site: Ovary. Species of origin Cricetulus griseus (Chinese hamster) (Cricetulus barabensis griseus) (NCBI Taxonomy: 10029) Hierarchy Parent: NTCC®_0214 (CHO-K1) Sex of cell Female Age at sampling Adult Category Spontaneously immortalized cell line β-Arrestin cell lines are stable clonal cell lines that expedite drug discovery and development by providing robust response to over 90% of all known G-protein coupled receptor (GPCRs), with accurate pharmacology. This assay measures an essential pathway in GPCR activation, i.e. β-arrestin recruitment to activated GPCRs, enabling scientists to screen for and profile functional agonists and inhibitors of GPCRs. These assays are successfully used to identify and optimize biologics and small molecule drugs, in addition to being used to develop potency assays for the QC lot release testing of numerous biologic drugs. Since β-arrestin recruitment occurs independent of G-protein coupling, these assays provide a direct, universal platform for measuring receptor activation. GPCR activation following ligand binding leads to β-arrestin recruitment to the receptor. This assay measures the activation status of the target GPCR by detecting β-arrestin recruitment using a homogeneous, easy-to-use, gain-of-signal assay based on Enzyme Fragment Complementation (EFC) technology. The PathHunter β-Arrestin GPCR Assay uses a β-galactosidase (β-gal) enzyme split into two fragments, the Enzyme Donor (ED) and Enzyme Acceptor (EA). Independently these fragments have no β-gal activity; however, in solution they can be brought together and complement to form an active β-gal enzyme. Here, the target GPCR is tagged with the small fragment of β-gal called ProLink™ (PK), a low affinity version of ED, and co-expressed in cells stably expressing β-Arrestin tagged with EA. Activation of the GPCR stimulates binding of β-arrestin to the ProLink-tagged GPCR, forcing complementation of PK and EA, resulting in the formation of an active β-gal enzyme. The resulting active enzyme hydrolyzes substrate present in the PathHunter detection reagents to generate light.

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