NTCC® cAMP Hunter™ CHO-K1 CALCRL-RAMP3 Gs Cell Line细胞株-BioVector NTCC典型培养物保藏中心

Name：NTCC® cAMP Hunter™ CHO-K1 CALCRL-RAMP3 Gs Cell Line
中文分类：NTCC® cAMP检测细胞株
Description描述：NTCC® cAMP Hunter Cell Lines.
Target靶点：CALCRL-RAMP3
ID编号:BioVector-95-0169C2
Size/Quantity数量: 2 Vials
Biosafety Level生物安全级别: 1
Shipping Info运输方式: Dry Ice
Storage储存方式: Liquid Nitrogen
Age年龄:
Gender性别:
Product Type细胞类型:
Reporter stable cell lines
Images图片:
Complete Growth Medium完全培养基: BioVector® cAMP Hunter™ CHO-K1 CALCRL-RAMP3 Gs Cell Line Specific Complete Medium
Subculturing传代方法:
Cryopreservation冻存方法:Freeze medium: Complete growth medium supplemented with 5% (v/v) DMSO

Storage temperature: liquid nitrogen vapor phase
Culture Conditions培养条件:Atmosphere: air, 95%; carbon dioxide (CO2), 5%

Temperature: 37.0°C
STR Profile鉴定数据:
Description描述：NTCC® GPCR cAMP and Calcium Cells - Ready-to-assay GPCR cryo-preserved cells validated for cAMP accumulation and calcium flux applications and ideal for screening of agonists, antagonists and various modulators.
NTCC® cAMP Hunter Gs cell lines overexpress naturally Gs coupled, wildtype GPCRs and are designed to detect increases in intracellular cAMP levels in response to agonist stimulation of the receptor. These cell lines are designed to be used in conjunction with the HitHunter® cAMP Assay detection kit.
GPCR cAMP Product Solutions
Homogeneous cAMP Detection Assay Solutions for Small Molecules and Biologics
cAMP cell-based assays measure cAMP levels in cells as a direct indication of GPCR functional status (activation or inhibition). This direct measurement is obtained by quantifying cellular cAMP accumulation levels in a dose-dependent manner with cell-based assays that are homogeneous and scalable.


NTCC® DiscoverX provides robust, easy-to-use, and high throughput screening (HTS) HitHunter® cAMP cell-based assays that accurately detect cellular cAMP levels in a variety of applications without the need for wash steps, optimization, or specialized equipment. These versatile assays provide a simple, validated detection system, optimized with over 125 cAMP Hunter™ cell lines, and include large signal-to-background, gain-of-signal responses. They can be used to monitor GPCR activation or inhibition with cAMP Hunter, ChemiSCREEN™, or non-Eurofins DiscoverX cell lines. Easily obtain reproducible results with high sensitivity, rank order ligands based on molecular potency, or determine pharmacological profiles of your ligands.


Used in the screening of millions of data points and referenced in hundreds of peer reviewed publications ranging from basic research to clinical applications, these assays provide accurate pharmacology of your ligand or small molecule and biologics while giving you the flexibility to work with multiple cell lines.

cAMP Product Highlights
Optional Formats – Select from hundreds of stable cell lines, frozen ready-to-assay cells, and assay ready eXpress and bioassay kits for chemiluminescent or fluorescent assay detection
Assay Ready Kits – Complete, ultra-convenient kits with optimized reagents for over 125 cAMP Hunter™ cell lines for monitoring GPCR activation using small molecules or biologics
High Throughput – Simple, rapid, and scalable assays that accurately detect cellular cAMP levels in 96-well through 3456-well formats
Validated Applications – Referenced products in over 400 peer reviewed publications ranging from basic research to clinical applications




Advantages of cAMP Product Solutions
Optimized for a Variety of Applications
Perform functional screens using small molecules or biologics (antibodies, peptides)
Determine pharmacological profiles and rank order ligands
Detect neutralizing antibodies (immunogenicity studies using serum and plasma)
Characterize phosphodiesterase inhibitors
HitHunter cAMP Assays
Eliminate optimization steps with an assay that has been designed and qualified for use with over 125 cAMP Hunter cell lines
Achieve precise characterization of ligand pharmacology with large assay windows, sensitive detection, and wide dynamic range for ligand bias studies and many more applications
Reproducible assay performance without fluorescence or serum interference and no need for specialized equipment
cAMP Hunter™ Cell Lines and Assay Ready Kits
Over 125 stable cell lines with unlimited culture and overexpressed naturally coupled, wild-type GPCRs
Ultra-convenient, complete eXpress kits for quick assessment without the need for cell culture
Ready-to-use bioassay kits provide cryopreserved cells and optimized reagents with high lot-to-lot reproducibility to support the lifetime of the biologic drug
ChemiSCREEN Cell Lines
Cell lines containing a promiscuous G protein, Gα15, to help funnel signaling to a common calcium readout, regardless of G protein coupling status
High functional expression of receptors with the ability to assay both agonist and antagonist in a single well
Stable cell lines for continuous culture and increased reliability and reproducibility assay results
Perform orthogonal assay confirmation such as second messenger signaling, e.g. cAMP, and ERK phosphorylation, for most targets
ChemiBRITE Cell Lines
Cell lines modified to flash with added brightness upon GPCR activation
Exceptional high signal-to-background ratio with no assay interference with autofluorescent compounds
Tool box capabilities allows generation of your own stable cell lines
ChemiSCREEN and ChemiBRITE Frozen Cells
Ready-to-Assay GPCR frozen cells come with plating media and enough cells to run either 96- or 384-well plate assays in full or half plate formats
Qualified for cAMP and calcium second messenger assays with results within 24 hours to accelerate your data generation
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Supplier供应商:BioVector质粒载体菌株细胞蛋白抗体基因保藏中心

NTCC典型培养物保藏中心

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