CHO-K1/ADRB2/Gα15 ADRB2 Stable Cell Line稳定表达细胞株 BioVector NTCC质粒载体菌种细胞基因保藏中心

CHO-K1/ADRB2/Gα15 ADRB2 Stable Cell Line稳定表达细胞株

Cell Line Name: CHO-K1/ADRB2/Gα15
Gene Synonyms: BAR; B2AR; ADRBR; ADRB2R; BETA2AR; ADRB2
Expressed Gene: Genbank Accession Number NM_000024; no expressed tags
Host Cell: CHO-K1/Gα15
Quantity: 2 vials (3×106
per vial) frozen cells
Stability: 16 passages
Application: Functional assay for ADRB2 receptor
Freeze Medium: 45% culture medium, 45% FBS, 10% DMSO
Complete Growth Medium: Ham’s F12, 10% FBS
Culture Medium: Ham’s F12, 10% FBS, 200 μg/ml Zeocin, 100 μg/ml Hygromycin B
Mycoplasma Status: Negative
Storage: Liquid nitrogen immediately upon delivery.
II. Background
β-Adrenergic receptors (β-ARs) are members of the superfamily G-protein-coupled receptors that are stimulated by
naturally occurring catecholamines, epinephrine, and norepinephrine. As part of the sympathetic nervous system,
β-ARs have been shown to have important roles in cardiovascular, respiratory, metabolic, central nervous system,
and reproductive functions. Three distinct β-AR subtypes have been identified (β1-AR, β2-AR, and β3-AR). All
three of these β-AR subtypes are believed to signal by coupling to the stimulatory G-protein Gsα which leads to the
activation of adenylyl cyclase and accumulation of the second messenger cAMP. β1-ARs mediate cardiac
stimulation, β2-ARs mediate smooth muscle relaxation in the peripheral vasculature and respiratory system, and
β3-AR has been shown to have important roles in adipose tissue and gastrointestinal tract. In studies using
subtype-selective agonists and antagonists in the human heart, β2-AR stimulation leads to the activation of
adenylyl cyclase and contributes to both inotropic and chronotropic responses.

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