艰难梭菌艰难梭菌VPI10463 ATCC43255 BioVector NTCC质粒载体菌种细胞基因保藏中心

艰难梭菌艰难梭菌VPI10463 ATCC43255

Clostridioides difficile strain VPI 10463 was isolated from an abdominal wound. This strain is toxigenic (toxinotype 0) and was confirmed by PCR to contain the tcdA and tcdB genes. The whole-genome sequenced bacterial strain has applications in enteric disease research and infectious disease research.

Product category
Bacteria
Product type
Anaerobe
Strain designation
VPI 10463
Type strain
No
Genome sequenced strain
Yes
Isolation source
Abdominal wound
Applications
Bioinformatics
Enteric disease research
Infectious disease research
Product format
Freeze-dried
Storage conditions
2°C to 8°C

General
Specific applications
Enteric Research
Preceptrol
No
Characteristics
Genotype
Toxinotype: 0
Toxigenic
Yes
Toxin genes
cdtB (Binary toxin) negative
tcdA (Toxin A) positive
tcdB (Toxin B) positive
Comments
Presence of tcdA and tcdB genes confirmed by PCR.
Ref
Toxinotype 0
Binary toxin gene cdtB not amplified by PCR
Ribotype 087
Toxin B production confirmed by cytotoxicity assay using Vero cells

Handling information
Medium
ATCC Medium 2107: Modified Reinforced Clostridial
ATCC Medium 260: Trypticase soy agar/broth with defibrinated sheep blood
ATCC Medium 5608: PY Medium (M2-VPI) With D-Glucose w/ Durham Tube
Temperature
37°C
Atmosphere
Anaerobic
Handling procedure
Open vial according to enclosed instructions.  Under anaerobic conditions, withdraw 0.5 mL of #2107 broth from a single test tube (5 to 6 mL) and rehydrate the entire vial contents.
Aseptically transfer this aliquot back into the broth. Additional tubes may be inoculated with 0.1 mL each from the suspension. 0.2 mL may also be inoculated onto a slant. Streak several blood plates to check for colonial morphology and purity.
Incubate tubes under an anaerobic atmosphere at 37°C.  Incubate one agar plate anaerobically for colony formation, and one aerobically for aerobic contamination check.
Within 24-48 hours, growth should be evident by turbidity in the broth and by colonies on the anaerobic agar surfaces.  No growth should occur on agar plates incubated aerobically.




ANAEROBIC CONDITIONS:

Anaerobic conditions for transfer may be obtained by either of the following:

Use of an anaerobic gas chamber, or
Placement of test tubes under a gassing cannula system connected to anaerobic gas.


Anaerobic conditions for incubation may be obtained by any of the following:

Loose screw caps on test tubes in anaerobic chamber,
Loose screw caps on test tubes in an activated anaerobic gas pack jar, or
Use of sterile butyl rubber stoppers on test tubes so that an anaerobic gas headspace is retained.
Handling notes
Anaerobe Systems Brucella Blood Agar plates (AS-111 or AS-141) can be used to analyze colony morphology and purity.
This item can also grow in #5608 broth.

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