MYA-2613 Yarrowia lipolytica strain菌株 BioVector NTCC质粒载体菌种细胞基因保藏中心

MYA-2613 Yarrowia lipolytica strain菌株

Yarrowia lipolytica (Wickerham et al.) van der Walt et von Arx
NTCC®MYA-2613™

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An ampoule containing viable cells (yeast cells, spores, or agar cubes with mycelia) suspended in cryoprotectant.    
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Product category
Fungi
Product type
Yeast
Strain designation
PO1f
Type strain
No
Genome sequenced strain
Yes
Isolation source
Derived from existing strain, W29
General
Specific applications
transformation host
Preceptrol
No
Characteristics
Genotype
MATA ura3-302 leu2-270 xpr2-322 axp2-deltaNU49 XPR2::SUC2
Morphology
On YEPD medium at 25°C after 5 days, colonies creamy white to buff, smooth becoming tomentose, margin entire becoming filamentous. Cells hyaline, subglobose to ovoid, smooth, 5.5-10.5 X 3-6 µm. Pseudohyphae present.
Comments
Unable to produce extracellular protease
Handling information
Medium
NTCC® Medium 28: Emmons' modification of Sabouraud's agar/broth
NTCC® Medium 200: YM agar or YM broth
NTCC® Medium 1245: YEPD
Temperature
24-26°C
Atmosphere
Aerobic
Handling procedure
Frozen ampoules packed in dry ice should either be thawed immediately or stored in liquid nitrogen. If liquid nitrogen storage facilities are not available, frozen ampoules may be stored at or below -70°C for approximately one week. Do not under any circumstance store frozen ampoules at refrigerator freezer temperatures (generally -20°C). Storage of frozen material at this temperature will result in the death of the culture.
To thaw a frozen ampoule, place in a 25°C to 30°C water bath, until just thawed (approximately 5 minutes).  Immerse the ampoule just sufficient to cover the frozen material.  Do not agitate the ampoule.
Immediately after thawing, wipe down ampoule with 70% ethanol and aseptically transfer at least 50 µl (or 2-3 agar cubes) of the content onto a plate or broth with medium recommended.
Incubate the inoculum/strain at the temperature and conditions recommended. Inspect for growth of the inoculum/strain regularly. The sign of viability is noticeable typically after 2-3 days of incubation. However, the time necessary for significant growth will vary from strain to strain.


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