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SNU869 cell line细胞株
Origin biliary tract
Species human
Cellular morphology epithelial
Growth pattern monolayer
Histopathology biliary tract cancer, ampulla of vater
Cell viability 91%
Freezing media RPMI1640, 52.5%; FBS, 40%; DMSO, 7.5%
Original media RPMI1640 with L-glutamine (300mg/L), 25mM HEPES and 25mM NaHCO3, 90%; heat inactivated fetal bovine serum (FBS), 10%
NTCC media RPMI1640 with L-glutamine (300mg/L), 25mM HEPES and 25mM NaHCO3, 90%; heat inactivated fetal bovine serum (FBS), 10%
Subculture Remove medium, add fresh 0.25 % trypsin 0.02 % EDTA solution, stand culture flask at 37''''C for 3 to 5 minutes, add culture medium and collect the cells, transfer the medium into 15ml tube, centrifuge, aspirate the medium, resuspend the pellets with culture medium and dispense into the culture flask
Populaton doubling time 48 hours
Split ratio 1:4
Media change Remove two thirds of medium and add fresh medium (depending on cell density) every 2 to 3 days
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