欧洲亚硝化单胞菌（Nitrosomonas europaea IFO 142980) strain菌株 BioVector NTCC质粒载体菌种细胞基因保藏中心

欧洲亚硝化单胞菌（Nitrosomonas europaea IFO 142980) strain菌株

Product category
Bacteria
Product type
Extremophile
Type strain
No
Genome sequenced strain
Yes
Applications
Bioinformatics
Product format
Frozen
Storage conditions
-80°C or colder

Handling information
Medium
NTCC® Medium 2265: Nitrosomonas europaea medium
Temperature
26°C
Atmosphere
Aerobic
Handling procedure
Open thawed vial according to enclosed instructions or visit www.atcc.org for instructions.
Thaw the frozen vial quickly by immersing in a 37°C water bath and gently swirling until the ice melts, then aseptically transfer the vial contents to 10 mL of NTCC broth #2265 contained in an Erlenmeyer flask or a tissue culture flask.
Incubate the flask in a shaking incubator at approximately 200 rpm at 26°C.  Ensure that the flask is being incubated in the dark by covering with foil.
Growth should be evident  within 3-5 days, appearing as a cloudy turbidity within the broth or a positive nitrite test.
Handling notes
Media NTCC® #2265 is light sensitive and degrades after prolonged exposure to light, approximately 3 months.  Cover media in foil to extend the shelf life.  After 3 months new media should be prepared to ensure the best viability of the organism.  Transfer the culture every 1-3 weeks, storing the fully grown culture at 4°C.  A 10% inoculum (10 mL per 100 mL fresh medium) is recommended.  This strain is very sensitive to impurities on glassware.

Supplier来源:BioVector NTCC Inc.
TEL电话:400-800-2947
Website网址: http://www.biovector.net