pLentiv2-dCas9-VP64 plasmid vector慢病毒基因编辑质粒载体 BioVector NTCC质粒载体菌种细胞基因保藏中心

pLentiv2-dCas9-VP64 plasmid vector慢病毒基因编辑质粒载体

(Empty Backbone) Expression of gRNA and dCas9-VP64 from the same plasmid for CRISPRa
BACKBONE
Vector backboneentiSAM v2 (Puro) addgene#92062
(Search Vector Database)
Backbone size (bp)15524
Modifications to backboneThe gRNA cloning site was replaced with the one from lentiCRISPR v2 (addgene# 52961)
Vector typeMammalian Expression, Lentiviral, CRISPR
Selectable markersPuromycin
GROWTH IN BACTERIA
Bacterial Resistance(s)Ampicillin, 100 μg/mL
Growth Temperature30°C
Growth Strain(s)NEB Stable
Copy numberUnknown
CLONING INFORMATION
Cloning methodRestriction Enzyme


Supplier来源:BioVector NTCC Inc.
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Website网址: http://www.biovector.net