SKW 6.4 cell line细胞株TIB-215 BioVector NTCC质粒载体菌种细胞基因保藏中心
- 价 格：¥98965
- 货 号：SKW 6.4 TIB-215
- 产 地：北京
- BioVector NTCC典型培养物保藏中心
- 联系人：Dr.Xu, Biovector NTCC Inc.
电话：400-800-2947 工作QQ:1843439339 (微信同号)
邮件：Biovector@163.com
手机：18901268599
地址：北京
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SKW 6.4 cell line细胞株TIB-215
Product categoryHuman cellsOrganismHomo sapiens, humanCell typeB lymphocyteMorphologylymphoblastApplications3D cell cultureImmunologyCharacteristicsGrowth propertiesSuspensionImmortalization methodEpstein-Barr virus (EBV) transformedGenes expressedimmunoglobulin, monoclonal antibodyIsotypeIgMCommentsThe cells respond to T Cell Replacing Factor (TRF) and B Cell Differentiation Factor (BCDF) by secreting IgM.Handling informationUnpacking and storage instructionsCheck all containers for leakage or breakage.Remove the frozen cells from the dry ice packaging and immediately place the cells at a temperature below _x001f_-130°C, preferably in liquid nitrogen vapor, until ready for use.Complete mediumThe base medium for this cell line is ATCC-formulated RPMI-1640 Medium, ATCC 30-2001. To make the complete growth medium, add the following components to the base medium: fetal bovine serum (ATCC 30-2020) to a final concentration of 10%.Temperature37°CAtmosphere95% Air, 5% CO2Handling procedureTo insure the highest level of viability, thaw the vial and initiate the culture as soon as possible upon receipt. If upon arrival, continued storage of the frozen culture is necessary, it should be stored in liquid nitrogen vapor phase and not at –70°C. Storage at –70°C will result in loss of viability.Thaw the vial by gentle agitation in a 37°C water bath. To reduce the possibility of contamination, keep the O-ring and cap out of the water. Thawing should be rapid (approximately 2 minutes).Remove the vial from the water bath as soon as the contents are thawed, and decontaminate by dipping in or spraying with 70% ethanol. All of the operations from this point on should be carried out under strict aseptic conditions.Transfer the vial contents to a 75 cm2 tissue culture flask and dilute with the recommended complete culture medium (see the specific batch information for the recommended dilution ratio). It is important to avoid excessive alkalinity of the medium during recovery of the cells. It is suggested that, prior to the addition of the vial contents, the culture vessel containing the growth medium be placed into the incubator for at least 15 minutes to allow the medium to reach its normal pH (7.0 to 7.6).Incubate the culture at 37°C in a suitable incubator. A 5% CO2 in air atmosphere is recommended if using the medium described on this product sheet.Note: If it is desired that the cryoprotective agent be removed immediately, or that a more concentrated cell suspension be obtained, centrifuge the cell suspension at approximately 125 x g for 5 to 10 minutes. Discard the supernatant and resuspend the cells with fresh growth medium at the dilution ratio recommended in the specific batch information.Subculturing procedureCultures can be maintained by addition of fresh medium. Alternatively, cultures can be established by centrifugation with subsequent resuspension at 5 x 105 viable cells/mL. Maintain cultures at a cell concentration between 4 x 105 and 2 x 106 cells/mL.Medium Renewal: Every 2 to 3 daysReagents for cryopreservationComplete growth medium supplemented with 5% (v/v) DMSO (ATCC 4-X)Quality control specificationsMycoplasma contaminationNot detectedSTR profilingAmelogenin: X,YCSF1PO: 11,12D13S317: 11D16S539: 11,12D5S818: 12,13D7S820: 9,11THO1: 8,9.3TPOX: 8,11vWA: 17,20
Supplier来源:BioVector NTCC Inc.
TEL电话:400-800-2947
Website网址: http://www.biovector.net
Product categoryHuman cellsOrganismHomo sapiens, humanCell typeB lymphocyteMorphologylymphoblastApplications3D cell cultureImmunologyCharacteristicsGrowth propertiesSuspensionImmortalization methodEpstein-Barr virus (EBV) transformedGenes expressedimmunoglobulin, monoclonal antibodyIsotypeIgMCommentsThe cells respond to T Cell Replacing Factor (TRF) and B Cell Differentiation Factor (BCDF) by secreting IgM.Handling informationUnpacking and storage instructionsCheck all containers for leakage or breakage.Remove the frozen cells from the dry ice packaging and immediately place the cells at a temperature below _x001f_-130°C, preferably in liquid nitrogen vapor, until ready for use.Complete mediumThe base medium for this cell line is ATCC-formulated RPMI-1640 Medium, ATCC 30-2001. To make the complete growth medium, add the following components to the base medium: fetal bovine serum (ATCC 30-2020) to a final concentration of 10%.Temperature37°CAtmosphere95% Air, 5% CO2Handling procedureTo insure the highest level of viability, thaw the vial and initiate the culture as soon as possible upon receipt. If upon arrival, continued storage of the frozen culture is necessary, it should be stored in liquid nitrogen vapor phase and not at –70°C. Storage at –70°C will result in loss of viability.Thaw the vial by gentle agitation in a 37°C water bath. To reduce the possibility of contamination, keep the O-ring and cap out of the water. Thawing should be rapid (approximately 2 minutes).Remove the vial from the water bath as soon as the contents are thawed, and decontaminate by dipping in or spraying with 70% ethanol. All of the operations from this point on should be carried out under strict aseptic conditions.Transfer the vial contents to a 75 cm2 tissue culture flask and dilute with the recommended complete culture medium (see the specific batch information for the recommended dilution ratio). It is important to avoid excessive alkalinity of the medium during recovery of the cells. It is suggested that, prior to the addition of the vial contents, the culture vessel containing the growth medium be placed into the incubator for at least 15 minutes to allow the medium to reach its normal pH (7.0 to 7.6).Incubate the culture at 37°C in a suitable incubator. A 5% CO2 in air atmosphere is recommended if using the medium described on this product sheet.Note: If it is desired that the cryoprotective agent be removed immediately, or that a more concentrated cell suspension be obtained, centrifuge the cell suspension at approximately 125 x g for 5 to 10 minutes. Discard the supernatant and resuspend the cells with fresh growth medium at the dilution ratio recommended in the specific batch information.Subculturing procedureCultures can be maintained by addition of fresh medium. Alternatively, cultures can be established by centrifugation with subsequent resuspension at 5 x 105 viable cells/mL. Maintain cultures at a cell concentration between 4 x 105 and 2 x 106 cells/mL.Medium Renewal: Every 2 to 3 daysReagents for cryopreservationComplete growth medium supplemented with 5% (v/v) DMSO (ATCC 4-X)Quality control specificationsMycoplasma contaminationNot detectedSTR profilingAmelogenin: X,YCSF1PO: 11,12D13S317: 11D16S539: 11,12D5S818: 12,13D7S820: 9,11THO1: 8,9.3TPOX: 8,11vWA: 17,20
Supplier来源:BioVector NTCC Inc.
TEL电话:400-800-2947
Website网址: http://www.biovector.net
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