BE257/pSGR3 coli strain核酸外切酶III生产菌株 BioVector NTCC质粒载体菌种细胞基因保藏中心

BE257/pSGR3 coli strain核酸外切酶III生产菌株

E. coli Exonuclease III purified from Escherichia coli BE257/pSGR3 is a 3' → 5' exonuclease specific for double-stranded
DNA. This enzyme has a strict specificity for double-stranded structures.
So, it is possible to delete from blunt end, 3'-recessed end and nicking
site, and it is impossible to delete from 3'-protruding end (Refer to Note).
So by the double digestion with two restriction enzymes which generate
different terminus respectively, the degradation from a single direction
is possible. The final product is single-stranded complementary DNA
and 5'-P mononucleotide. As the base dependence of this enzyme is low
and the reaction rarely stops at GC rich site, this enzyme is suitable for
construction of DNA deletion.

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