pIC44 plasmid vector整合型质粒载体 BioVector NTCC质粒载体菌种细胞基因保藏中心

pIC44 plasmid vector整合型质粒载体

Construction of integrative vector pIC44. The chloramphenicol resistance gene of plasmid pRF30 was introduced into Bluescript SK, giving plasmid pSKCm. This plasmid was digested with KpnI and XhoI and ligated to a DNA fragment containing part of the 16S and 23S rRNA genes of the rrnB operon of R. fascians. Ap, ampicillin resistance gene; Cm, chloramphenicol resistance gene; MCS, multiple cloning site; ori, replication origin of the ColE1 plasmid.

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