A2780cis cell line人卵巢癌顺铂耐药细胞株 BioVector NTCC质粒载体菌种细胞基因保藏中心

A2780cis cell line人卵巢癌顺铂耐药细胞株

Cell Line Name: NTCC® A2780cis

Keywords: Human ovarian carcinoma
Cell Line Description: This cisplatin-resistant cell line has been developed by chronic exposure of the parent cisplatin-sensitive A2780 cell line (NTCC® 93112519) to increasing concentrations of cisplatin. A2780cis is cross-resistant to melphalan, adriamycin and irradiation. An increased ability to repair DNA damage as well as cytogenetic abnormalities has been observed. In order to retain resistance cisplatinum has to be added to the media for every passage. In addition to this matched pair of drug-sensitive/resistant cell lines an adriamycin-resistant cell line, A2780adr (NTCC®. 93112520), has been isolated from the same parental line A2780.
Species: Human
Tissue of Origin: ovary
CellType: Epithelial
Growth Mode: Adherent

Karyotype: Modal no. 46

Subculture Routine: Split sub-confluent cultures (70-80%) 1:3 to 1:6 i.e. seeding at 2-4 x10,000cells/cm² using 0.05% trypsin or trypsin/EDTA; 5% CO2; 37°C. Cells will attach slowly after resuscitation and take up to 7 days to reach confluency. Recommendation: resuscitate cells in media without cisplatin. Add after subculture of attached cells.
Culture Medium: RPMI 1640 + 2mM Glutamine + 1µM cisplatinum + 10% Foetal Bovine Serum (FBS) .

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