Mm.cargo[SpCas9] plasmid vector质粒载体 BioVector NTCC质粒载体菌种细胞基因保藏中心

Mm.cargo[SpCas9] plasmid vector质粒载体

Expresses mouse Peg10 cargoRNA encoding SpCas9

BACKBONE
Vector backbonepcDNA3.1
(Search Vector Database)
Backbone size w/o insert (bp)5300
Total vector size (bp)10581
Vector typeMammalian Expression
GROWTH IN BACTERIA
Bacterial Resistance(s)Ampicillin, 100 μg/mL
Growth Temperature37°C
Growth Strain(s)NEB Stable
Copy numberHigh Copy
GENE/INSERT
Gene/Insert nameSpCas9 flanked by MmPeg10 UTRs
SpeciesM. musculus (mouse); S.pyogenes
Insert Size (bp)5200
PromoterCMV
Tags / Fusion Proteins
HA-SV40 NLS (N terminal on insert)
SV40 NLS (C terminal on insert)
CLONING INFORMATION
Cloning methodGibson Cloning
5′ sequencing primerCMV Forward

Supplier来源:BioVector NTCC Inc.
TEL电话:400-800-2947
Website网址: http://www.biovector.net