CAG-GFP-IRES-CRE plasmid vector逆病毒荧光重组质粒载体 BioVector NTCC质粒载体菌种细胞基因保藏中心

CAG-GFP-IRES-CRE plasmid vector逆病毒荧光重组质粒载体

retroviral vector encoding for GFP and Cre-recombinase
BACKBONE
Vector backboneCAG-GFP
(Search Vector Database)
Backbone manufacturerGage lab (Addgene plasmid# 16664)
Backbone size w/o insert (bp)6764
Total vector size (bp)9328
Modifications to backbonevector backbone based on the pCL system by Naviaux et al.,J. Virol. 70, 5701–5705 (1996).
Vector typeMammalian Expression, Retroviral
GROWTH IN BACTERIA
Bacterial Resistance(s)Ampicillin, 100 μg/mL
Growth Temperature37°C
Growth Strain(s)Top10
Copy numberHigh Copy
GENE/INSERT 1
Gene/Insert nameeGFP
Alt nameEnhanced Green Fluorescent Protein
Alt nameN/A
Alt nameN/A
SpeciesAequorea victoria green
Insert Size (bp)700
GenBank ID
PromoterCAG
CLONING INFORMATION FOR GENE/INSERT 1
Cloning methodUnknown
5′ sequencing primerCTC GGC AAC GTG CTG GTT GTT
3′ sequencing primerCAACATAGTTAAGAATACCAGTC
(Common Sequencing Primers)
GENE/INSERT 2
Gene/Insert nameCre
Alt nameCre-recombinase
SpeciesM. musculus (mouse)
Insert Size (bp)991
GenBank ID
Promoterlinked by IRES
CLONING INFORMATION FOR GENE/INSERT 2
Cloning methodUnknown
5′ sequencing primerIRES-F
3′ sequencing primerWPRE-R

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