pTARBAC1 plasmid vector细菌人工染色体质粒载体 BioVector NTCC质粒载体菌种细胞基因保藏中心

pTARBAC1 plasmid vector细菌人工染色体质粒载体

a new hybrid vector,
pTARBAC1, containing a yeast artificial chromosome (YAC) cassette (a yeast selectable
marker and a centromere). The cassette allows transferring of BACs into yeast for their further modification. Furthermore, the new hybrid vector provides the opportunity to re-isolate
each DNA insert without construction of a new library of random clones. Digestion of a BAC
DNA by an endonuclease that has no recognition site in the vector, but which deletes most
of the internal insert sequence and leaves the unique flanking sequences, converts a BAC into
a TAR vector, thus allowing direct gene isolation. Cotransformation of a TAR vector and
genomic DNA into yeast spheroplasts, and subsequent recombination between the TAR vector’s flanking ends and a specific genomic fragment, allows rescue of the fragment as a circular YAC/BAC molecule. Here we prove a new cloning strategy by re-isolation of randomly
chosen genomic fragments of different size from T. brucei cloned in BACs.

Supplier来源:BioVector NTCC Inc.
TEL电话:400-800-2947
Website网址: http://www.biovector.net