Syn61Δ3(ev5) ΔrecA (ev1) strain大肠杆菌突变菌株 BioVector NTCC质粒载体菌种细胞基因保藏中心

Syn61Δ3(ev5) ΔrecA (ev1) strain大肠杆菌突变菌株

Laboratory adapted, genomically recoded E. coli strain without TCG, TCA, or TAG codons and deleted serT, serU, prfA, and recA genes. Evolved for ~270 generations in LB at 37 °C.

GROWTH IN BACTERIA
Bacterial Resistance(s)Streptomycin, 50 μg/mL
Growth Temperature37°C
Growth Strain(s)Syn61Δ3(ev5) ΔrecA (ev1)
Growth instructionsGrow in LB, SOB, or 2×YT at 37°C
Copy numberUnknown
GENE/INSERT
Gene/Insert nameΔrecA
SpeciesSynthetic
MutationΔrecA
CLONING INFORMATION
Cloning methodUnknown
5′ sequencing primerGAGTTTACGTCGCAGTTCTTG
3′ sequencing primerACTGAAAGCGGCTCGTGCT

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