Ureaplasma urealyticum strain菌株27814 BioVector NTCC质粒载体菌种细胞基因保藏中心

Ureaplasma urealyticum strain菌株27814

Ureaplasma urealyticum Shepard et al.
27814™

Ureaplasma urealyticum strain 23 was isolated from a patient with nongonococcal urethritis.

Product category
Bacteria
Strain designation
23
Type strain
No
Isolation source
Nongonococcal urethritis
Product format
Freeze-dried
Storage conditions
2°C to 8°C

Characteristics
Serotype
II
Handling information
Medium
ATCC Medium 1092: B-broth medium for Ureaplasma
Temperature
37°C
Atmosphere
Aerobic
Handling procedure
Follow instructions as suggested for the culturing of Mollicutes:
PROCEDURES FOR PROPAGATING MOLLICUTES:

Open the vial according to the enclosed instructions.
Using a Pasteur or 1.0 mL pipette, withdraw approximately 0.5 to 1.0 mL from a tube containing 2.5 mL of #1092 broth. Rehydrate the entire pellet.
Aseptically transfer this aliquot back into the tube.  Mix well.
Make serial dilutions by transferring 0.25 mL from the original tube to a tube containing 2.25 mL.  Repeat process by transferring 0.25 mL from the second to a third tube, etc.  Dilutions are important, not only for titration purposes, but also to keep culture in varying stages of growth.  Many strains will die out rapidly once acid or alkaline conditions are reached.  It is recommended to prepare several dilutions from the initial tube as the cryoprotectant used in the freeze-drying process often inhibits growth.
Incubate all tubes and the purity plate at 37°C aerobically.
Depending on the medium used, growth will be indicated by increased turbidity, a color change, or both.
Handling notes
This strain requires an additional 10% horse serum (ThermoFisher, 16050122) for growth.

Ureaplasmas grow very rapidly.  The indicator in the first tube will change color to a darker green within hours.  It is especially important to make serial dilutions of this strain, for when alkaline conditions are reached (as indicated by the color change), the culture will rapidly die out unless refrigerated immediately (+4°C) or stored frozen at -60°C.  Refrigerated broth cultures may remain viable for periods up to 30 days.  No visible turbidity will be seen.  The color change is the only indication of growth.  Therefore, transfer, freeze, or lyophilize the culture as soon as possible.  Growth on agar medium is very poor and slow.  Broth is the best method for propagation.

Store vials at freezer temperatures until ready to use.

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