永生化人牙齦角質形成細胞Immortalized Human Gingival Keratinocytes BioVector NTCC质粒载体菌种细胞基因保藏中心

永生化人牙齦角質形成細胞Immortalized Human Gingival Keratinocytes

The gingival epithelium protects periodontal tissue from bacteria and toxic substances. Keratinocytes play an important role in promoting strong epithelial bonds through the formation of intercellular connections with the cytoskeleton and neighboring cells. The Immortalized Human Gingival Keratinocytes are isolated primary oral keratinocytes transfected to express the HPV-16 E7. It expresses cytokeratin 2, cytokeratin 17, cytokeratin 19, involucrin, filaggrin, and claudin-1. These cells may be differentiated by altering culturing conditions to build transepithelial electrical resistance, a distinct characteristic of gingival keratinocytes. These cells are recommended for studies in mechanisms that contribute to the specific properties of the gingiva tissue, such as the epithelial barrier function.
Species
Homo sapiens
Source
Buccal Gingiva
Culture Properties
Adherent
Morphology
Polygonal
Immortalization Method
Serial passaging and chemical mediated transfection with plasmid carrying human papilloma virus (HPV-16) E6 and E7 genes


The base medium for this cell line is NTCC-formulated Eagle's Minimum Essential Medium, To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.

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