HHV-4 virus病毒 BioVector NTCC质粒载体菌种细胞基因保藏中心

HHV-4 virus病毒

The Epstein-Barr virus (EBV or HHV-4) is a member of the herpesvirus family. It shares common traits with the viruses that cause chickenpox, shingles, cold sores, and genital herpes. EBV is tremendously prevalent—approximately 90–95% of all adults in the United States have previously been infected with EBV

Human gammaherpesvirus 4 (HHV-4)
Cat No.: BioVector®-VR1482

Human gammaherpesvirus 4 (HHV-4) strain B95-8 was isolated from human lymphoblastoid cell line (883L) derived from a patient with transfusion-induced infectious mononucleosis.
Product category
Viruses
Classification
Herpesviridae, Lymphocryptovirus
Strain designation
B95-8
Common name
Epstein-Barr virus
Isolation source
Human lymphoblastoid cell line (883L) derived from a patient with transfusion-induced infectious mononucleosis.
Product format
Frozen
Shipping information
5 x 1.0 mL vials
Storage conditions
-70°C or colder

General
Specific applications
(1984), the B95-8 cells used to produce this strain of EBV are infected with and may also shed an uncharacterized type D retrovirus.
Characteristics
Comments
A 1 mL aliquot of this EBV preparation is capable of transforming B-lymphocytes from a preparation of normal human lymphocytes when co-cultivated with a feed layer culture (such as NTCC® 55-X). VR-1492 is the designation for the NTCC® set of five 1 mL vials of VR-1491. VR-1491 is EBV virus shed in the supernatant of persistently infected and transformed B95-8 cells. The EBV virus itself is of human origin. The B95-8 cells used to produce this strain of EBV are infected with and may also shed an uncharacterized type D retrovirus.

Quality control testing is done by transformation assay instead of TCID [50]
Mycoplasma contamination
Not detected
Handling information
Host
B95-8 cells
Effects
CPE; cell transformation (cone-shaped cells, cells with spike-like projections, and small clusters of healthy cells)
Complete medium
RPMI 1640 (NTCC® 30-2001) + 10% FBS (NTCC® 30-2020)
Temperature
37°C
Atmosphere
95% Air, 5% CO2
Incubation
10-25 days
Recommendation for infection
Incubate in a humidified 5% CO2 atmosphere until indicators of successful transformation appear. Incubation: 10-25 days
Key abbreviations
°C, Degrees Celsius
CO2, Carbon dioxide
CPE, Cytopathic effect
EBV, Epstein-Barr virus
FBS, Fetal bovine serum

Supplier来源:BioVector NTCC Inc.
TEL电话:400-800-2947
Website网址: http://www.biovector.net