pBP-T7RNAP Plasmid 原核表达质粒74096 BioVector NTCC质粒载体菌种细胞基因保藏中心

pBP-T7RNAP Plasmid 原核表达质粒74096

PURPOSE
T7 RNA polymerase PCR cloned from BL21 (DE3) genomic DNA

BACKBONE
Vector backbonepSB1C3
(Search Vector Database)
Backbone manufactureriGEM
Backbone size w/o insert (bp)2044
Total vector size (bp)4743
Modifications to backboneModifications between XbaI and PstI from original pSB1C3 plasmid. CAT gene - C435G (nucleotide) - silent mutagenesis to remove BsmBI site
Vector typeBacterial Expression, Synthetic Biology
GROWTH IN BACTERIA
Bacterial Resistance(s)Chloramphenicol, 25 μg/mL
Growth Temperature37°C
Growth Strain(s)DH5alpha
Copy numberHigh Copy
GENE/INSERT
Gene/Insert nameT7 RNAP
Alt nameT7 RNA polymerase
SpeciesEnterobacteria phage T7
Insert Size (bp)2652
GenBank IDGU071091.1
PromoterNone
CLONING INFORMATION
Cloning methodRestriction Enzyme
5′ cloning siteBsaI (not destroyed)
3′ cloning siteBsaI (not destroyed)
5′ sequencing primertgccacctgacgtctaagaa; gaatacgctgaggctatcgcaac
3′ sequencing primerattaccgcctttgagtgagc; cttagtgcccagcttgactttctc

Supplier来源:BioVector NTCC Inc.
TEL电话:400-800-2947
Website网址: http://www.biovector.net