pCMV_hyPBase plasmid vector质粒载体 BioVector NTCC质粒载体菌种细胞基因保藏中心

pCMV_hyPBase plasmid vector质粒载体

The generation of a hyperactive mutant of PBase. We first established a yeast assay to efficiently screen for mutations in the PBase, which give rise to hyperactivity. Individual candidate mutants were then verified for their transposition activity in mouse ES cells. Finally, we combined all the mutations into one sequence and generated a hyperactive PBase (hyPBase), which shows more than 10-fold higher rates of transposition than the WT mammalian codon-optimized PBase (mPBase).

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