pORANGE Cloning template vector plasmid vector质粒载体 BioVector NTCC质粒载体菌种细胞基因保藏中心

pORANGE Cloning template vector plasmid vector质粒载体

PURPOSE
(Empty Backbone) Cloning template form ORANGE method based knock-in constructs

pORANGE
(Search Vector Database)
Backbone size (bp)8494
Vector typeMammalian Expression
Tag / Fusion Protein
HA (N terminal on insert)
GROWTH IN BACTERIA
Bacterial Resistance(s)Ampicillin, 100 μg/mL
Growth Temperature37°C
Growth Strain(s)DH5alpha
Copy numberHigh Copy
GENE/INSERT
Gene/Insert nameSpCas9
SpeciesS. pyogenes
PromoterU6 and Cbh
Tag / Fusion Protein
HA (N terminal on insert)
CLONING INFORMATION
Cloning methodRestriction Enzyme
5′ cloning siteUnknown (unknown if destroyed)
3′ cloning siteUnknown (unknown if destroyed)
5′ sequencing primeraaggctgttagagagataattggaa
3′ sequencing primercgggccatttaccgtaagtt

Supplier来源:BioVector NTCC Inc.
TEL电话:400-800-2947
Website网址: http://www.biovector.net