hTERT PF179T CAF cell line人永生化肿瘤成纤维细胞株 BioVector NTCC质粒载体菌种细胞基因保藏中心
- 价 格：¥98965
- 货 号：hTERT PF179T CAF
- 产 地：北京
- BioVector NTCC典型培养物保藏中心
- 联系人：Dr.Xu, Biovector NTCC Inc.
电话：400-800-2947 工作QQ:1843439339 (微信同号)
邮件：Biovector@163.com
手机：18901268599
地址：北京
- 已注册
hTERT PF179T CAF cell line人永生化肿瘤成纤维细胞株
hTERT PF179T CAFNTCC®CRL-3290Product categoryHuman cellsProduct typehTERT-immortalized cellOrganismHomo sapiens, humanCell typefibroblastTissueProstateDiseaseCancerApplications3D cell cultureCancer researchDrug developmentHigh-throughput screeningGeneralSpecific applicationsProstate cancer biology or tumor microenvironment studyCharacteristicsCells per vialApproximately 1.0 x 106Volume1.0 mLGrowth propertiesAdherentGenderMaleKaryotypeabnormal, loss of Y chromosomeHandling informationUnpacking and storage instructionsCheck all containers for leakage or breakage.Remove the frozen cells from the dry ice packaging and immediately place the cells at a temperature below _x001f_-130°C, preferably in liquid nitrogen vapor, until ready for use.Complete mediumThe base medium for this cell line is Eagle's Minimal Essential Medium (EMEM; ATCC® 30-2003™). To make the complete medium add the following components to 500 mL of the base medium:56 mL fetal bovine serum (FBS; ATCC® 30-2020™) for a final concentration of 10%5.0 mL sodium bicarbonate 7.5% solution (GE Life Sciences Hyclone SH30033.01)56 µL puromycin (10 mg/mL; Gibco cat# A11138-03) for a final concentration of 1 µg/mLTemperature37°CAtmosphere95% Air, 5% CO2Handling procedureTo ensure the highest level of viability, thaw the vial and initiate the culture as soon as possible upon receipt. If upon arrival, continued storage of the frozen culture is necessary, it should be stored in liquid nitrogen vapor phase and not at -70°C. Storage at -70°C will result in loss of viability. Thaw the vial by gentle agitation in a 37°C water bath. To reduce the possibility of contamination, keep the O-ring and cap out of the water. Thawing should be rapid (approximately 2 minutes).Remove the vial from the water bath as soon as the contents are thawed, and decontaminate by dipping in or spraying with 70% ethanol. All of the operations from this point on should be carried out under strict aseptic conditions.Transfer the vial contents to a centrifuge tube containing 9.0 mL complete culture medium. and spin at approximately 125 x g for 5 to 7 minutes.Resuspend cell pellet with the recommended complete medium (see the specific batch information for the culture recommended dilution ratio). It is important to avoid excessive alkalinity of the medium during recovery of the cells. It is suggested that, prior to the addition of the vial contents, the culture vessel containing the complete growth medium be placed into the incubator for at least 15 minutes to allow the medium to reach its normal pH (7.0 to 7.6). pH (7.0 to 7.6).Incubate the culture at 37°C in a suitable incubator. A 5% CO2 in air atmosphere is recommended if using the medium described on this product sheet. Subculturing procedureVolumes used in this protocol are for 75 cm2 flask; proportionally reduce or increase amount of dissociation medium for culture vessels of other sizes. Corning® T-75 flasks (catalog #430641) are recommended for subculturing this product.Remove and discard culture medium.Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.Add 2.0 to 3.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gently pipetting.Add appropriate aliquots of the cell suspension to new culture vessels.Cultures can be established between 2 x 104 and 4 x 104 viable cells/cm2.Incubate cultures at 37°C.Interval: Maintain cultures at a cell concentration between 2 X 104 and 3.2 X 105 cell/cm2.Subcultivation Ratio: A subcultivation ratio of 1:3 to 1:8 is recommendedMedium Renewal: 2 to 3 times per week
Supplier来源:BioVector NTCC Inc.
TEL电话:400-800-2947
Website网址: http://www.biovector.net
hTERT PF179T CAFNTCC®CRL-3290Product categoryHuman cellsProduct typehTERT-immortalized cellOrganismHomo sapiens, humanCell typefibroblastTissueProstateDiseaseCancerApplications3D cell cultureCancer researchDrug developmentHigh-throughput screeningGeneralSpecific applicationsProstate cancer biology or tumor microenvironment studyCharacteristicsCells per vialApproximately 1.0 x 106Volume1.0 mLGrowth propertiesAdherentGenderMaleKaryotypeabnormal, loss of Y chromosomeHandling informationUnpacking and storage instructionsCheck all containers for leakage or breakage.Remove the frozen cells from the dry ice packaging and immediately place the cells at a temperature below _x001f_-130°C, preferably in liquid nitrogen vapor, until ready for use.Complete mediumThe base medium for this cell line is Eagle's Minimal Essential Medium (EMEM; ATCC® 30-2003™). To make the complete medium add the following components to 500 mL of the base medium:56 mL fetal bovine serum (FBS; ATCC® 30-2020™) for a final concentration of 10%5.0 mL sodium bicarbonate 7.5% solution (GE Life Sciences Hyclone SH30033.01)56 µL puromycin (10 mg/mL; Gibco cat# A11138-03) for a final concentration of 1 µg/mLTemperature37°CAtmosphere95% Air, 5% CO2Handling procedureTo ensure the highest level of viability, thaw the vial and initiate the culture as soon as possible upon receipt. If upon arrival, continued storage of the frozen culture is necessary, it should be stored in liquid nitrogen vapor phase and not at -70°C. Storage at -70°C will result in loss of viability. Thaw the vial by gentle agitation in a 37°C water bath. To reduce the possibility of contamination, keep the O-ring and cap out of the water. Thawing should be rapid (approximately 2 minutes).Remove the vial from the water bath as soon as the contents are thawed, and decontaminate by dipping in or spraying with 70% ethanol. All of the operations from this point on should be carried out under strict aseptic conditions.Transfer the vial contents to a centrifuge tube containing 9.0 mL complete culture medium. and spin at approximately 125 x g for 5 to 7 minutes.Resuspend cell pellet with the recommended complete medium (see the specific batch information for the culture recommended dilution ratio). It is important to avoid excessive alkalinity of the medium during recovery of the cells. It is suggested that, prior to the addition of the vial contents, the culture vessel containing the complete growth medium be placed into the incubator for at least 15 minutes to allow the medium to reach its normal pH (7.0 to 7.6). pH (7.0 to 7.6).Incubate the culture at 37°C in a suitable incubator. A 5% CO2 in air atmosphere is recommended if using the medium described on this product sheet. Subculturing procedureVolumes used in this protocol are for 75 cm2 flask; proportionally reduce or increase amount of dissociation medium for culture vessels of other sizes. Corning® T-75 flasks (catalog #430641) are recommended for subculturing this product.Remove and discard culture medium.Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.Add 2.0 to 3.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gently pipetting.Add appropriate aliquots of the cell suspension to new culture vessels.Cultures can be established between 2 x 104 and 4 x 104 viable cells/cm2.Incubate cultures at 37°C.Interval: Maintain cultures at a cell concentration between 2 X 104 and 3.2 X 105 cell/cm2.Subcultivation Ratio: A subcultivation ratio of 1:3 to 1:8 is recommendedMedium Renewal: 2 to 3 times per week
Supplier来源:BioVector NTCC Inc.
TEL电话:400-800-2947
Website网址: http://www.biovector.net
- 公告/新闻
免费订购电话: 400-800-2947