Clostridium butyricum strain丁酸梭菌ATCC19398 BioVector NTCC质粒载体菌种细胞基因保藏中心
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Clostridium butyricum strain丁酸梭菌ATCC19398
Clostridium butyricum Prazmowski NCTC 7423Cat No.: NTCC®-19398Clostridium butyricum strain NCTC 7423 [IFO 13949, VPI 3266] was isolated from the intestine of a pig.Product categoryBacteriaProduct typeAnaerobeStrain designationNCTC 7423 [IFO 13949, VPI 3266]Type strainYesIsolation sourceIntestine of pigImage:Handling informationMediumNTCC Medium 2107: Modified Reinforced ClostridialNTCC Medium 260: Trypticase soy agar/broth with defibrinated sheep bloodTemperature37°CAtmosphereAnaerobicHandling procedureOpen thawed vial according to enclosed instructions.Under anaerobic conditions aseptically transfer the entire contents to a 5-6 mL tube of #2107 broth. Additional test tubes can be inoculated by transferring 0.5 mL of the primary broth tube to these secondary broth tubes. Best practice dictates the use of pre-reduced media.Use several drops of the primary broth tube to inoculate a #260 plate and/or #260 agar slant.Incubate in an anaerobic atmosphere at 37°C for 24-48 hours. Incubate one agar plate aerobically at 37°C to check for contamination.ANAEROBIC CONDITIONS:Anaerobic conditions for transfer may be obtained by the use of an anaerobic gas chamber or placement of test tubes under a gassing cannula system connected to anaerobic gas.Anaerobic conditions for incubation may be obtained by any of the following:Loose screw caps on test tubes in an anaerobic chamberLoose screw caps on test tubes in an activated anaerobic gas pack jarUse of sterile butyl rubber stoppers on test tubes so that an anaerobic gas headspace is retainedHandling notesThis item may need to have growth established first in broth for 24 hours before culturing on agar.Anaerobe Systems Brucella Blood plate (AS-111 or AS-141) can be used to analyze colony morphology and purity.Always use freshly prepared pre-reduced media or pre-reduced media that has been previously prepared but stored under anaerobic conditions. Resazurin in the media is a color indicator for anaerobic conditions. Observance of pink color in medium before use or during incubation shows anaerobic conditions have not been met and oxidation has occurred. Medium should be discarded.HistoryDeposited asClostridium butyricum PrazmowskiDepositorsNCTCChain of custodyNTCC<-- NCTC <-- J Howie strain RowettType of isolateAnimalCross referencesGenBank M59085 Clostridium butyricum 16S ribosomal RNA.GenBank U09980 Clostridium butyricum NTCC 19398 Spo0A (spo0A) gene, partial cds.
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Clostridium butyricum Prazmowski NCTC 7423Cat No.: NTCC®-19398Clostridium butyricum strain NCTC 7423 [IFO 13949, VPI 3266] was isolated from the intestine of a pig.Product categoryBacteriaProduct typeAnaerobeStrain designationNCTC 7423 [IFO 13949, VPI 3266]Type strainYesIsolation sourceIntestine of pigImage:Handling informationMediumNTCC Medium 2107: Modified Reinforced ClostridialNTCC Medium 260: Trypticase soy agar/broth with defibrinated sheep bloodTemperature37°CAtmosphereAnaerobicHandling procedureOpen thawed vial according to enclosed instructions.Under anaerobic conditions aseptically transfer the entire contents to a 5-6 mL tube of #2107 broth. Additional test tubes can be inoculated by transferring 0.5 mL of the primary broth tube to these secondary broth tubes. Best practice dictates the use of pre-reduced media.Use several drops of the primary broth tube to inoculate a #260 plate and/or #260 agar slant.Incubate in an anaerobic atmosphere at 37°C for 24-48 hours. Incubate one agar plate aerobically at 37°C to check for contamination.ANAEROBIC CONDITIONS:Anaerobic conditions for transfer may be obtained by the use of an anaerobic gas chamber or placement of test tubes under a gassing cannula system connected to anaerobic gas.Anaerobic conditions for incubation may be obtained by any of the following:Loose screw caps on test tubes in an anaerobic chamberLoose screw caps on test tubes in an activated anaerobic gas pack jarUse of sterile butyl rubber stoppers on test tubes so that an anaerobic gas headspace is retainedHandling notesThis item may need to have growth established first in broth for 24 hours before culturing on agar.Anaerobe Systems Brucella Blood plate (AS-111 or AS-141) can be used to analyze colony morphology and purity.Always use freshly prepared pre-reduced media or pre-reduced media that has been previously prepared but stored under anaerobic conditions. Resazurin in the media is a color indicator for anaerobic conditions. Observance of pink color in medium before use or during incubation shows anaerobic conditions have not been met and oxidation has occurred. Medium should be discarded.HistoryDeposited asClostridium butyricum PrazmowskiDepositorsNCTCChain of custodyNTCC<-- NCTC <-- J Howie strain RowettType of isolateAnimalCross referencesGenBank M59085 Clostridium butyricum 16S ribosomal RNA.GenBank U09980 Clostridium butyricum NTCC 19398 Spo0A (spo0A) gene, partial cds.
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