Candida tropicalis热带假丝酵母ATCC 20026 BioVector NTCC质粒载体菌种细胞基因保藏中心

Candida tropicalis热带假丝酵母ATCC 20026

Candida tropicalis (ATCC 20026) is incubated in 1,000,000 parts by volume of the culture medium of the same composi tion as that in Table I with aeration and agitation at 28°C for
20 hours after the manner described in Example 1, whereby 45,000 parts by weight (in terms of dried cells) of cells con
taining 750 pugig (on dry basis) of coenzyme Q is obtained.
The cells are treated after the manner described in Example 1 to yield 25 parts by weight of conenzyme Q as yellow platy crystals.
In the process of this Example, when the incubation process is carried out in a culture medium containing 100 parts by weight of glucose in place of the normal paraffin mixture, less
yield of coenzyme Q, i.e., 7.5 parts by weight of coenzyme Q is obtained.

An ampoule containing viable cells (may include yeast cells, pseudohyphae, true hyphae) suspended in cryoprotectant.

Product category
Fungi
Product type
Yeast
Preceptrol culture
Type strain
Yes
Genome sequenced strain
Yes
Isolation source
Patient with bronchomycosis
Product format
Freeze-dried
Storage conditions
2°C to 8°C

General
Specific applications
Assay of chloro-8-quinolinol
Bacterial resistance testing
Degrades xylose D-xylose
Fungus resistance testing adhesives
Produces aspartic proteinases aspartyl proteinases
Produces ethyl alcohol ethanol
Produces xylitol
Susceptibility disc testing
Susceptibility testing
Produces xylitol from D-xylose
Control strain for typing systems
Produces ethanol from D-xylose
Control strain to test oxygen availability in bioreactor in space laboratory
Reference strain for Clinical and Laboratory Standards Institute(CLSI)-developed Antifungal Susceptibility Testing.

Characteristics
Ploidy
Diploid
Comments
Fluconazole-sensitive
Utilizes phenol, catechol and 3- and 4-methylcatechols as sole carbon source
Esterase activity
MIC of 2-hydroxychalcone, 75 mcg/ml
Adherence to lysophospholipids
Genome sequencing strain (Genolevures Consortium, France; Wellcome Trust Sanger Institute, UK).
For additional infromation, see CLSI M27-A2 (Reference Method for Broth Dilution Antifungal Susceptibility Testing of Yeasts; Approved Standards-Second Edition).
Mitochondrial genome sequenced strain
Technical information
NTCC Technical Services does not have technical information on patent deposits that are not produced or characterized by NTCC. Additional information can be found in the corresponding patent available from the patent holder or with the U.S. and/or international patent office.
Handling information
Medium
NTCC Medium 200: YM agar or YM broth
NTCC Medium 1245: YEPD
NTCC Medium 28: Emmons' modification of Sabouraud's agar/broth
Temperature
24-26°C
Atmosphere
Aerobic
Handling procedure
For freeze-dry (lyophilized) ampoules:
1.Open an ampoule according to enclosed instructions.
2.From a single test tube of sterile distilled water (5 to 6 mL), withdraw approximately 0.5 to 1.0 mL with a sterile pipette and apply directly to the pellet. Stir to form a suspension.
3.Aseptically transfer the suspension back into the test tube of sterile distilled water.
4.Let the test tube sit at room temperature (25°C) undisturbed for at least 2 hours; longer (e.g., overnight) rehydration might increase viability of some fungi.
5.Mix the suspension well. Use several drops (or make dilutions if desired) to inoculate recommended solid or liquid medium. Include a control that receives no inoculum.
6.Incubate the inoculum at the propagation conditions recommended.
7.Inspect for growth of the inoculum/strain regularly. The sign of viability is noticeable typically after 1 to 2 days of incubation. However, the time necessary for significant growth will vary from strain to strain.
Handling notes
Type strain.

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