Mycoplasma pneumoniae Strain FH肺炎支原体15531 BioVector NTCC质粒载体菌种细胞基因保藏中心

Mycoplasma pneumoniae Strain FH肺炎支原体15531

Mycoplasma pneumoniae Strain FH
Cat No.: NTCC-015531

Mycoplasma pneumoniae FH strain of Eaton Agent [NCTC 10119] is a whole genome-sequenced type strain strain and the first Mycoplasma known to be the etiological agent of a human disease. This strain was isolated by Hayflick from monkey kidney tissue-culture fluids of the FH strain (Eaton Agent Virus) supplied by C. Liu, who recovered this strain in embryonated eggs from a student with atypical pneumonia. This can be used in media testing, respiratory research, and pharmaceutical and personal care.

Product category
Bacteria
Strain designation
FH strain of Eaton Agent [NCTC 10119]
Type strain
Yes
Genome sequenced strain
Yes
Isolation source
Isolated by Hayflick from monkey kidney tissue-culture fluids of the FH strain (Eaton Agent Virus) supplied by C. Liu, who recovered this strain in embryonated eggs from a student with atypical pneumonia.
Applications
Infectious disease research
Respiratory disease research
Media testing
Quality control
Pharmaceutical testing
Product format
Freeze-dried
Storage conditions
2°C to 8°C

Specific applications
Media testing
Respiratory research
Pharmaceutical and Personal Care
Recommended as a test microorganism for EP 2.6.7 Mycoplasmas
Recommended as a test microorganism for JP 16 G3 Biotechnological/Biological Products: Mycoplasma Testing for Cell Substrates Used for the Production of Biotechnological/Biological Products
Recommended as a test microorganism for USP <63> Mycoplasma Tests

Characteristics
Comments
First mycoplasma known to be the etiological agent of a human disease

Handling information
Medium
NTCC Medium 988: Spiroplasma medium SP-4
NTCC Medium 2611: Spiroplasma Medium - Special Modified Formulation
Temperature
37°C
Atmosphere
Broth: Aerobic
Plates: 5% CO2 or candle jar
Handling procedure
Follow instructions as suggested for the culturing of Mollicutes:
            PROCEDURES FOR PROPAGATING MOLLICUTES:
Add an additional 5% heat-inactivated fetal bovine serum to medium #988. Open the vial according to the enclosed instructions.
Using a Pasteur or 1.0 mL pipette, withdraw approximately 0.5 to 1.0 mL from a T-flask containing 2.5 mL of the recommended broth. Rehydrate the entire pellet.
Aseptically transfer this aliquot back into the tube. Mix well.
Make serial dilutions by transferring 0.25 mL from the original tube to a tube containing 2.5 mL. Repeat process by transferring 0.25 mL from the second to a third tube, etc. Dilutions are important, not only for titration purposes, but also to keep culture in varying stages of growth. Many strains will die out rapidly once acid or alkaline conditions are reached. It is recommended to prepare several dilutions from the initial tube as the cryoprotectant used in the freeze drying process often inhibits growth.
Use an uninoculated tube of broth to serve as a control.
Plates may be inoculated to check colony morphology. You can also spot each dilution on the surface of plate (4 or more/plate) to determine the number of colony-forming units. Growth on agar may take up to 2 weeks when grown at 37°C in 5% CO2.  

2.Mycoplasma pneumoniae strains are very slow growing and produce a very light turbidity. Growth in broth is best observed after 10 to 14 days of incubation. It usually takes at least seven days for the first T-flasks to start showing growth. Growth is easily recognized by an indicator change from red to orange to yellow. The cells are best transferred when the medium is orange. After medium changes to yellow, cells have started to die.
3.For long term storage of Mycoplasma pneumoniae, freeze-drying or freezing is recommended. Liquid nitrogen storage is the best method. Optimally grown cells are centrifuged at 9000 rpm for 30 minutes, the supernatant poured off, and the packed cells resuspended in a smaller amount of #2611 broth. To this, add an equal amount of sterile 20% glycerol as a cryoprotectant. This suspension is aliquoted into small plastic vials and stored at -70°C or colder.

Handling notes
Growth may take up to 2 weeks.
Commercially available SP4 Glucose (Remel catalog # R112585 or #R20376 for broth, and Remel catalog # R20276 for agar) may yield the best growth.
This strain requires an additional 5% heat-_x001f_inactivated fetal bovine serum be added to NTCC Medium #988 or #2611 or SP4 Glucose broths to sustain growth of this strain.
The flasks should be checked to make sure media is not evaporating and to add media as needed to maintain the volume.
We have found that using a candle jar for CO2 conditions works better for those strains whose medium has an indicator present. CO2 incubators may lower the pH of the medium enough to cause a color change. This change may make it difficult to observe growth with those strains that show little turbidity.
Purifed genomic DNA of this strain is available as NTCC® 015531D.

Quality control specifications
Verification method
Whole-genome Sequencing

Supplier来源:BioVector NTCC Inc.
TEL电话:400-800-2947
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