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FLK-BLV cell line细胞株
Cell line:FLK-BLVNTCC no.:ACC 153Species validation:ovine (sheep) (Ovis aries)Cell type:embryonal kidney cellsOrigin:established from the embryonal kidney cells of an ovine in 1976; cells are susceptible to infection with BLV (bovine leukemia virus) leading to the formation of syncythiaRisk assessment:The cell line is infected with bovine leukemia virus (BLV) and produces active viruses. The viruses are not pathogenic to humans, but may be pathogenic to animals. NTCC Cell Culture Data:Morphology:large fibroblast-like cells growing as monolayers; Medium:90% MEM (with Earle's salts) + 10% h.i. FBS optional: + 850 mg/L NaHCO3 + 120 mg/L sodium pyruvate + 1x non-essential amino acidsSubculture:seed out about 1-2 x 106 cells/80 cm2; split confluent culture 1:2 to 1:8 every 3-5 days using trypsin/EDTAIncubation:at 37 °C with 5% CO2Doubling time:ca. 50 hoursHarvest:cell harvest of about 4 x 106/80 cm2Storage:frozen with 82.5% medium, 10% FBS, 7.5% DMSO NTCC Scientific Data:Mycoplasma:negative in DAPI, microbiological culture, RNA hybridization, PCR assaysSpecies validation:Species-level identification was carried out by mitochondrial Cytochrome C Oxidase Subunit 1 (COI) DNA barcoding according standard ANSI/ATCC ASN-0003-2015 and revealed Ovis aries species.Viruses:ELISA: reverse transcriptase positive; PCR: SMRV -
Supplier来源:BioVector NTCC Inc.
TEL电话:400-800-2947
Website网址: http://www.biovector.net
Cell line:FLK-BLVNTCC no.:ACC 153Species validation:ovine (sheep) (Ovis aries)Cell type:embryonal kidney cellsOrigin:established from the embryonal kidney cells of an ovine in 1976; cells are susceptible to infection with BLV (bovine leukemia virus) leading to the formation of syncythiaRisk assessment:The cell line is infected with bovine leukemia virus (BLV) and produces active viruses. The viruses are not pathogenic to humans, but may be pathogenic to animals. NTCC Cell Culture Data:Morphology:large fibroblast-like cells growing as monolayers; Medium:90% MEM (with Earle's salts) + 10% h.i. FBS optional: + 850 mg/L NaHCO3 + 120 mg/L sodium pyruvate + 1x non-essential amino acidsSubculture:seed out about 1-2 x 106 cells/80 cm2; split confluent culture 1:2 to 1:8 every 3-5 days using trypsin/EDTAIncubation:at 37 °C with 5% CO2Doubling time:ca. 50 hoursHarvest:cell harvest of about 4 x 106/80 cm2Storage:frozen with 82.5% medium, 10% FBS, 7.5% DMSO NTCC Scientific Data:Mycoplasma:negative in DAPI, microbiological culture, RNA hybridization, PCR assaysSpecies validation:Species-level identification was carried out by mitochondrial Cytochrome C Oxidase Subunit 1 (COI) DNA barcoding according standard ANSI/ATCC ASN-0003-2015 and revealed Ovis aries species.Viruses:ELISA: reverse transcriptase positive; PCR: SMRV -
Supplier来源:BioVector NTCC Inc.
TEL电话:400-800-2947
Website网址: http://www.biovector.net
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