pcDNA-hDLL1 gene基因表达质粒敲除编辑载体 BioVector NTCC质粒载体菌种细胞基因保藏中心

pcDNA-hDLL1 gene基因表达质粒敲除编辑载体

pcDNA-hDLL1

RefSeq NM_005618.4
RefSeq Size 3366 bp
RefSeq ORF 2172 bp
Locus ID 28514
Domains DSL, EGF_CA, EGF, EGF
Protein Families Adult stem cells, Cancer stem cells, ES Cell Differentiation/IPS, Stem cell relevant signaling - DSL/Notch pathway, Transmembrane
Protein Pathways Notch signaling pathway
MW 77.9 kDa
Gene Summary DLL1 is a human homolog of the Notch Delta ligand and is a member of the delta/serrate/jagged family. It plays a role in mediating cell fate decisions during hematopoiesis. It may play a role in cell-to-cell communication.
启动子
Enhanced CMV promoter
载体
BioVector® pcDNA
限制性酶切位点
KpnI + XbaI(6.1kb+2.17kb)
测序引物
T7( 5' TAATACGACTCACTATAGGG 3' )
BGH( 5' TAGAAGGCACAGTCGAGG 3' )
质控
The plasmid is confirmed by full-length sequencing.
筛选
细菌筛选抗性
Ampicillin
细胞筛选抗性
Hygromycin
应用
Stable or Transient mammalian expression


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