EHSPEL Mouse chondrosarcoma cell line小鼠软骨肉瘤细胞株 BioVector NTCC质粒载体菌种细胞基因保藏中心
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- 货 号：EHSPEL
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EHSPEL Mouse chondrosarcoma cell line小鼠软骨肉瘤细胞株
Cell line name: NTCC® EHSPELSynonyms EHS parietal Endoderm-LikeDisease Mouse chondrosarcoma (NCIt: C24018)Species of origin Mus musculus (Mouse) (NCBI Taxonomy: 10090)Breed/subspecies: ST/Eh.Originate from same individual: BAM [Mouse chondrosarcoma]EHSCategory Cancer cell lineImage:Description:Engelbreth-Holm-Swarm (EHS) tumor produces large amounts of basement membrane (BM) components, which are widely used as cell culture substrates mimicking BM functions. EHS tumor arose spontaneously in an ST/Eh strain mouse and has been propagated by transplantation. In the present study, we established a cell line, NTCC® EHSPEL (EHS Parietal Endoderm-Like), which can be cultured ex vivo and preserves the capacity to form tumors in vivo. NTCC® EHSPEL cells secreted large amounts of laminin-1 into the medium and deposited BM components onto dishes. To further characterize NTCC® EHSPEL cells, their gene expression profile was compared to those of parietal endoderm cells from Reichert's membrane at embryonic day 13.5, differentiated F9 embryonal carcinoma cells, and PYS-2 parietal endoderm cells. These analyses outlined not only common features of parietal endoderm-like cells that underlie the efficient production of BM components, but also germline cell-like features of NTCC® EHSPEL cells, at least some of which may play crucial roles in their capacity to form tumors that accumulate abundant BM components in vivo. Karyotyping of NTCC® EHSPEL cells using chromosome painting probes showed a large number of interchromosomal rearrangements and partial chromosome hyperploidy. Exogenous introduction of a human laminin-alpha(4)-EGFP fusion protein into NTCC® EHSPEL cells resulted in the production and deposition of human-mouse-hybrid laminin-8. This strategy should be applicable for creating efficient systems to produce chimeric laminins as well as BM-like gels with modified biological activity.NTCC® EHSPEL Complete Medium
Supplier来源:BioVector NTCC Inc.
TEL电话:400-800-2947
Website网址: http://www.biovector.net
Cell line name: NTCC® EHSPELSynonyms EHS parietal Endoderm-LikeDisease Mouse chondrosarcoma (NCIt: C24018)Species of origin Mus musculus (Mouse) (NCBI Taxonomy: 10090)Breed/subspecies: ST/Eh.Originate from same individual: BAM [Mouse chondrosarcoma]EHSCategory Cancer cell lineImage:Description:Engelbreth-Holm-Swarm (EHS) tumor produces large amounts of basement membrane (BM) components, which are widely used as cell culture substrates mimicking BM functions. EHS tumor arose spontaneously in an ST/Eh strain mouse and has been propagated by transplantation. In the present study, we established a cell line, NTCC® EHSPEL (EHS Parietal Endoderm-Like), which can be cultured ex vivo and preserves the capacity to form tumors in vivo. NTCC® EHSPEL cells secreted large amounts of laminin-1 into the medium and deposited BM components onto dishes. To further characterize NTCC® EHSPEL cells, their gene expression profile was compared to those of parietal endoderm cells from Reichert's membrane at embryonic day 13.5, differentiated F9 embryonal carcinoma cells, and PYS-2 parietal endoderm cells. These analyses outlined not only common features of parietal endoderm-like cells that underlie the efficient production of BM components, but also germline cell-like features of NTCC® EHSPEL cells, at least some of which may play crucial roles in their capacity to form tumors that accumulate abundant BM components in vivo. Karyotyping of NTCC® EHSPEL cells using chromosome painting probes showed a large number of interchromosomal rearrangements and partial chromosome hyperploidy. Exogenous introduction of a human laminin-alpha(4)-EGFP fusion protein into NTCC® EHSPEL cells resulted in the production and deposition of human-mouse-hybrid laminin-8. This strategy should be applicable for creating efficient systems to produce chimeric laminins as well as BM-like gels with modified biological activity.NTCC® EHSPEL Complete Medium
Supplier来源:BioVector NTCC Inc.
TEL电话:400-800-2947
Website网址: http://www.biovector.net
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