pSI Mammalian Expression Vector哺乳动物表达载体质粒 BioVector NTCC质粒载体菌种细胞基因保藏中心

pSI Mammalian Expression Vector哺乳动物表达载体质粒

Description
The pCI and pSI Mammalian Expression Vectors are designed to promote constitutive expression of cloned DNA inserts in mammalian cells. The major difference between the two vectors is the enhancer/promoter region controlling the expression of the inserted gene. The pSI Expression Vector contains the simian virus 40 (SV40) enhancer and early promoter region, whereas the pCI Expression Vector contains the human cytomegalovirus (CMV) major immediate-early gene enhancer/promoter region. The pSI and pCI Vectors can be used for both transient and stable expression of genes. For stable expression, the pSI or pCI Vectors must be co-transfected with an expression vector containing a selectable gene for mammalian cells.
Use T7 EEV Promoter Primer (Cat.# BioVector-Q6700) to sequence ssDNA produced by the pCI and pSI Vectors. Do Not use the T7 Promoter Primer (Cat.# BioVector-Q5021) to sequence the pCI or pSI Vectors. There is a sequence difference between the primer and the promoter sequences.

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