pBHG-fiber35 plasmid腺病毒骨架质粒载体 BioVector NTCC质粒载体菌种细胞基因保藏中心

pBHG-fiber35 plasmid腺病毒骨架质粒载体

A modified adenoviral skeleton plasmid, comprising a viral gene replication complex which is gene sequences encoding viral gene replication-related proteins nsP1, nsP2, nsP3 and nsP4, wherein the adenoviral skeleton plasmid is selected from pAdEasy-1, pAdEasy-2, pBHG11, pBHG-fiber5, or pBHG-fiber35.
Preferably, the recombinant viral vector is obtained by co-transfecting a modified adenoviral skeleton plasmid and a shuttle plasmid containing an antigen gene. The modified adenoviral skeleton plasmid includes a viral gene replication complex, which is derived from proteins related to replication of coding virus genes of RNA viruses, and more preferably, the viral gene replication complex is nucleotide sequences of nsP1, nsP2, nsP3 and nsP4 from alphaviruses. The adenoviral skeleton plasmid is selected from pAdEasy-1, pAdEasy-2, pBHG11, pBHG-fiber5, or pBHG-fiber35.

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