pBHG35 plasmid vector腺病毒骨架质粒载体 BioVector NTCC质粒载体菌种细胞基因保藏中心

pBHG35 plasmid vector腺病毒骨架质粒载体

The product was cloned into siRNA vector pDC316-EGFP-U6. The insertion plasmid pDC316-PI3KcbsiRNA-EGFP was identified by PCR, and then DNA sequencing demonstrated the correct orientation and sequence. The reaction product was transformed into E. col i (DH5α), and the positive bacterial colony was selected. The recombinant eukaryotic expression vector pDC316-PI3KcbsiRNA-EGFP was identified by using restriction endonucleases digestive reaction and DNA sequencing. The HEK 293 cells were cotransfected by the shuttle plasmid of pDC316-PI3KcbsiRNA and the skeleton plasmid of pBHG35, and the recombinant plasmid of Ad5F35-PI3KcbsiRNA was obtained. The expression of the transfected genes was evaluated by PCR and immunocytochemical stain. Virus was purified by double CsCl centrifugation and subsequently dialysed as described previously

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