大肠杆菌 DSM2304 strain菌株/感受态细胞-BioVector NTCC质粒载体菌株细胞蛋白抗体基因保藏中心

Product category产品种类: Bacteria
Classification分类: Enterobacteriaceae, Escherichia
菌株名称Strain designation: 大肠杆菌 DSM2304
描述Discription: Name: Escherichia coli (Migula 1895) Castellani and Chalmers 1919 DSM No.: 2304 Strain designation: K12 M5004, M5004 Country: country of origin unknown Date of sampling: before 03.03.1982 Nagoya Protocol Restrictions: There are NO known Nagoya Protocol restrictions for this strain. History: <- W. Bode <- B.J. Bachmann, K12 M5004 Cultivation conditions: Medium 381 plus 0.2% glucose, 37°C标准菌株Type strain: No
提供形式Product format: Freeze-dried
保存温度Storage conditions: 2°C to 8°C

操作方法Handling information
培养基Medium: BioVector® Medium 3: Nutrient agar or nutrient broth
培养温度Temperature: 37°C
气体环境Atmosphere:Aerobic

操作流程Handling procedure
Open vial according to enclosed instructions.
Using a single tube of #3 broth (5 to 6 mL), withdraw approximately 0.5 to 1.0 mL with a Pasteur or 1.0 mL pipette. Rehydrate the entire pellet.
Aseptically transfer this aliquot back into the broth tube. Mix well.
Use several drops of the suspension to inoculate a #3 agar slant and/or plate
Incubate the tubes and plate at 37°C for 24 hours.