广宿主质粒pDVN-1,pSC259, pCHR71, R751, pJRD215, pMC903 strain菌株/感受态细胞-BioVector NTCC质粒载体菌株细胞蛋白抗体基因保藏中心

Product category产品种类: Bacteria
Classification分类: Enterobacteriaceae, Escherichia
菌株名称Strain designation: 广宿主质粒pDVN-1,pSC259, pCHR71, R751, pJRD215, pMC903
描述Discription: pDVN-1 Apr, source of the Tn3 inverted repeat New et al. pSC259 Derivative of pSC101 (Kmr tnpA) Owen et al. pCHR71 IncW R338 rep (Ts) Tmpr Tra+ Sasakawa and Yoshikawa R751 IncPl Tmpr Tra+ J. A. Shapiro pJRD184 Apr Tcr Heusterspreute et al. pJRD215 IncP4 Kmr Smr Davison et al. pMC903 pACYC177::lacZY M. Casadaban pSPORT1 lacIq lacPOZa 标准菌株Type strain: No
提供形式Product format: Freeze-dried
保存温度Storage conditions: 2°C to 8°C

操作方法Handling information
培养基Medium: BioVector® Medium 3: Nutrient agar or nutrient broth
培养温度Temperature: 37°C
气体环境Atmosphere:Aerobic

操作流程Handling procedure
Open vial according to enclosed instructions.
Using a single tube of #3 broth (5 to 6 mL), withdraw approximately 0.5 to 1.0 mL with a Pasteur or 1.0 mL pipette. Rehydrate the entire pellet.
Aseptically transfer this aliquot back into the broth tube. Mix well.
Use several drops of the suspension to inoculate a #3 agar slant and/or plate
Incubate the tubes and plate at 37°C for 24 hours.