NTCC's Schwann Cell Medium人施旺细胞专用培养基 BioVector NTCC质粒载体菌种细胞基因保藏中心

NTCC's Schwann Cell Medium人施旺细胞专用培养基

NTCC's Schwann Cell Medium
Cat# NTCC-SCM

NTCC's Schwann Cell Medium (SCM), when used with Schwann Cell Growth Supplement (SCGS, Cat #NTCC-M1752) and 25 ml of fetal bovine serum (FBS) is a complete medium designed for optimal growth of normal schwann cells in vitro. It is a sterile, liquid medium which contains essential and non-essential amino acids, vitamins, organic and inorganic compounds, hormones, growth factors, trace minerals and a low concentration of fetal bovine serum (5%). The medium is HEPES and bicarbonate buffered and has a pH of 7.4 when equilibrated in an incubator with an atmosphere of 5% CO2/95% air. The medium is formulated (quantitatively and qualitatively) to provide an optimally balanced nutritional environment that selectively supports the growth of normal schwann cells in vitro.
Country of Manufacture BioVector NTCC Inc. United States
Product Code NTCC-SCM
Size/Quantity 500 ml
Product Use This product is for research use only. It is not approved for use in humans, animals, or in vitro diagnostic procedures.
Storage Store the basal medium at 4°C, the SCGS, the FBS and the P/S solution at -20°C. Protect from light.
Shipping Info Basal medium: room temperature. Supplements: dry ice.

Components
NTCC's SCM consists of 500 ml of basal medium,25 ml of FBS(Cat. #NTCC-M0025),5 ml of Schwann Cell Growth Supplement(SCGS,Cat.#NTCC-M1752) and 5 ml of penicillin/streptomycin solution (P/S,Cat.#NTCC-M0503).Note:FBS, SCGS and PIS are not pre-mixed in SCM; they must be added separately to make the complete SCM.

Product Use
SCM is for rescarch use only. It is not approved for human or animal use, or for application in in vitro diagnostic procedures.

Storage
Store the basal medium at 4Cand the SCGS,FBS and P/S solution at -20°C. Protect from light.

Shipping
Basal medium: room temperature.Supplements: dry ice.

Instructions for Use
Thaw SCGS,FBS and P/S solution at 37C.Gently tilt the tubes several times to ensure the contents are completely mixed before adding to the medium.Spray the medium bottle and tubes with 70%ethanol, and wipe to remove excess liquid. In a sterile field,remove the caps without touching the interior threads with fingers.Add sCGS,FBS and P/S solution to the medium and mix well.Since several components are light-labile, the medium should not be exposed to light for extended periods.We do not recommend warming medium in a 37C water bath prior to use. When stored in the dark at 4°C, the reconstituted medium is stable for one month.

Supplier来源:BioVector NTCC Inc.
TEL电话:400-800-2947
Website网址: http://www.biovector.net