pCT-tRNA plasmid热带念珠菌基因编辑质粒载体 BioVector NTCC质粒载体菌种细胞基因保藏中心
- 价 格:¥49865
- 货 号:pCT-tRNA
- 产 地:北京
- BioVector NTCC典型培养物保藏中心
- 联系人:Dr.Xu, Biovector NTCC Inc.
电话:400-800-2947 工作QQ:1843439339 (微信同号)
邮件:Biovector@163.com
手机:18901268599
地址:北京
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pCT-tRNA plasmid热带念珠菌基因编辑质粒载体
CRISPR-Cas9 system for genetic manipulation of Candida tropicalis
The plasmid contains the following relevant inserts: 1. C. albicans Autonomously Replicating Sequence 2 for episomal propagation in C. tropicalis; 2. CAS9 sequence expressed from a Meyerozyma guilliermondii promoter; 3. Cassette for the expression of the sgRNA from a Ashbya gossypii RNA pol II promoter. The mature sgRNA is released following RNase Z processing of the upstream tRNA and self-cleavage of the dowstream HDV Ribozyme. The guide targeting the gene of interest can be cloned into the SapI/BspQI digested plasmid. The regulatory elements (species of origin of promoters/terminators) are described in the map.
Vector type CRISPR
Selectable markers SAT1 (resistance to Nourseothricin)
Bacterial Resistance(s)Ampicillin, 100 μg/mL
Growth Temperature 37°C
Growth Strain(s)DH5alpha
Copy numberHigh Copy
Supplier来源:BioVector NTCC Inc.
TEL电话:400-800-2947
Website网址: http://www.biovector.net
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