首页 » pCT-tRNA plasmid热带念珠菌基因编辑质粒载体 BioVector NTCC质粒载体菌种细胞基因保藏中心

pCT-tRNA plasmid热带念珠菌基因编辑质粒载体 BioVector NTCC质粒载体菌种细胞基因保藏中心

  • 价  格:¥49865
  • 货  号:pCT-tRNA
  • 产  地:北京
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pCT-tRNA plasmid热带念珠菌基因编辑质粒载体

CRISPR-Cas9 system for genetic manipulation of Candida tropicalis

The plasmid contains the following relevant inserts: 1. C. albicans Autonomously Replicating Sequence 2 for episomal propagation in C. tropicalis; 2. CAS9 sequence expressed from a Meyerozyma guilliermondii promoter; 3. Cassette for the expression of the sgRNA from a Ashbya gossypii RNA pol II promoter. The mature sgRNA is released following RNase Z processing of the upstream tRNA and self-cleavage of the dowstream HDV Ribozyme. The guide targeting the gene of interest can be cloned into the SapI/BspQI digested plasmid. The regulatory elements (species of origin of promoters/terminators) are described in the map.

Vector type CRISPR
Selectable markers SAT1 (resistance to Nourseothricin)
Bacterial Resistance(s)Ampicillin, 100 μg/mL
Growth Temperature 37°C
Growth Strain(s)DH5alpha
Copy numberHigh Copy

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