TA1535/pSK1002 BioVector’s Salmonella typhimurium strain肠道沙门氏菌环境水质检测AMES检测用菌-BioVector NTCC Inc.

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The multicopy plasmid BioVector^TM pSK1002, a fusion product of genes of theumu operon (which was derived

from E. coli K12 AB1157) with the vector pMC1403 (a pBR322 derivative), carries a umuC'-lacZ gene fusion

product. The umu operon is regulated by the SOS genes recA (protease) and lexA (repressor) and consists of a

promoter, operator,umuD and umuC genes. The vector carries the lacZ and lacY genes, the ampicillin resistance

gene and ori. The strain BioVector^TMTA1535 (AMES test strain) is uvrB (deficient in excision repair), rfa (incomplete cell wall

causing good permeability) and does not possess a chromosomallacZ gene. pSK1002 makes the strain

selectable, and the umuCgene product (UmuC protein) functions as a mutator protein. The umutest is a

short-term genotoxicity assay for the detection of mutagenic potentials of chemicals etc.

Mol. weight:

15 kb

Marker:

Apr

Distributed in:

Salmonella enterica subsp. enterica TA1535 (Salmonella typhimurium)

Cultivation conditions: TGA-MEDIUM . 37° C

TGA-MEDIUM:

Tryptone (Bacto) 10.0 g Glucose 2.0 g NaCl 5.0 g Distilled water 1000.0 ml Adjust pH to 7.0.

The SOS/umu assay is used to evaluate the ability of testing substance or sample to induce DNA damage. The system is

based on alterations in the induction of SOS response as a consequence of DNA damage. SOS/umu assay is convenient

as a screening assay for large number of samples as it is a fast and simple method. The test is standardised for the

determination of the genotoxic potential of water and wastewater. (ISO/DIS 13829, 2000. Determination of genotoxicity of

water and wastewater using the umu-test. TC 147/SC 5.)

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