E.coli K12菌株衍生物A19 strain菌株 BioVector NTCC质粒载体菌种细胞基因保藏中心

E.coli  K12菌株衍生物A19 strain菌株

Sex(Hfr,F+,F-,or F'): Hfr
Hfr Point of Origin#: 21
PO Map Position: 97.00

Direction of Marker Transfer: Clockwise

6 Mutations:
Mutation Location Certainty Display
rna-19 13.87 1 rna-19
gdhA2 39.67 1 gdhA2
his-95 45.01 0 his-95
relA1 62.71 1 relA1
spoT1 82.34 1 spoT1
metB1 88.94 1 metB1

Comments:
The his marker may have reverted.
The strain has been reported by Reiner to have an unidentified growth requirement satisfied by small amounts of yeast extract.

References:
Clark, A.J. 1963. Genetic analysis of a "double male" strain of Escherichia coli K-12. Genetics 48:105-120
Gesteland, RF 1966. Isolation and characterization of ribonuclease I mutants of Escherichia coli. J. Mol. Biol. 16(1):67-84.
Reiner, AM 1969. Genetic locus for ribonuclease I in Escherichia coli. J. Bacteriol. 97(3):1522-3.

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