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HDLM2 cell line人霍奇金氏淋巴瘤细胞株-BioVector NTCC质粒载体菌种细胞基因保藏中心

  • 价  格:¥98965
  • 货  号:HDLM2
  • 产  地:北京
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HDLM2 cell line人霍奇金氏淋巴瘤细胞株


Cell line:
HDLM-2
NTCC no.:
rNTCC®ACC 17
Species validation:
human (Homo sapiens)
Cell type:
Hodgkin lymphoma
Origin:
established from the pleural effusion of a 74-year-old man with Hodgkin lymphoma (nodular sclerosing, stage IV) in 1982. Exome and RNA sequence data are available (see Ref 18187 and Exome sequence and RNA-Seq)


Biosafety level:
1
Permissions and restrictions:
A

NTCC Cell Culture Data:
Morphology:
single, round to polygonal cells in suspension; heterogeneity in cell size, 15-50 µm; many bi- and polynucleated cells; image; image; image; image; image
Medium:
80% RPMI 1640 + 20% h.i. FBS
Subculture:
seed out at ca. 1 x 106 cells/ml in a 24-well-plate; maintain between 0.5-1.5 x 106 cells/ml; split carefully, 1:2 every 2-3 days
Incubation:
at 37 °C with 5% CO2
Doubling time:
about 70-75 hours
Harvest:
maximal density of 2.0-2.5 x 106 cells/ml
Storage:
frozen with 70% medium, 20% FBS, 10% DMSO

NTCC Scientific Data:
Mycoplasma:
contamination was initially eliminated with Ciprobay (ciprofloxacin), thereafter negative in DAPI, microbiological culture, RNA hybridization and repeatedly in PCR assays
Immunology:
CD2 +, CD3 -, CD4 (+), CD13 -, CD14 -, CD15 +, CD19 -, CD21 -, CD25 +, CD30 +, HLA-DR +; image
Authenticity:
STR analysis according to the global standard ANSI/ATCC ASN-0002.1-2021 (2021) resulted in an authentic STR profile of the reference STR database.
Cytogenetics:
remarkable human hypodiploid cell line with large number of rearrangements involving all chromosomes except ch 10 - 36(35-38)<2n>X, der(Y), breakpoints at: 1p21, 1q11, 2p14, 2q37, 3q21, 3p14, 3q11, 3q25.2, 3q27, 4q25, 5q11, 5q35, 6q15/16, 7p14, 8q21.1, 8q23, 8q24.2, 9p11, 11q13, 12p12, 12q24.3, 13p11, 13q14, 14q21, 14q23/24.2, 15p11, 15q13, 17p11, 17q25, 18p11, 19p13, 19q13, 21q21, 22q33, Ycen, Yq12
Viruses:
ELISA: reverse transcriptase negative; PCR: EBV -, HBV -, HCV -, HHV-8 -, HIV-1 -, HIV-2 -, HTLV-1/2 -, MLV -, SMRV -

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