pP22-sec短芽孢杆菌分泌型表达载体，适合高表达真核生物基因 BioVector NTCC质粒载体菌种细胞基因保藏中心

pP22-sec短芽孢杆菌分泌型表达载体，适合高表达真核生物基因

pP22-sec is an efficient vector for producing secreted proteins and
is ideal for eukaryotic recombinant protein expression.Brevibacillus choshinensis is a grampositive bacterium well-suited for heterologous protein expression and is capable of producing
high yields of active protein. This system offers the following advantages:
・Extracellular secretion of large volumes of protein
・Simple procedures for genetic manipulation and culturing
・Almost no detectable protease activity
・Production of active protein
・Safe host bacteria
Examples of protein production using this system are shown in Table 1. High expression of
biologically active protein (enzymes, antigens, and cytokines) was obtained regardless of gene
origin (bacterial, archaea, or eukaryotic). In particular, eukaryotic secretory proteins often contain
disulfide (S-S) bonds and are generally difficult to produce in prokaryotic expression systems. The
B. choshinensis Host-Vector System can produce high yields of secretory proteins, including those
with disulfide bonds.

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