Propionibacterium acidipropionici strain费氏丙酸杆菌菌株4875 BioVector NTCC质粒载体菌种细胞基因保藏中心

Propionibacterium acidipropionici strain费氏丙酸杆菌菌株4875

Propionibacterium acidipropionici 费氏丙酸杆菌
Cat# BioVector4875

Product category
Bacteria
Strain designation
4 [VPI 0402]
Type strain
No
Isolation source
Dairy products
Product format
Freeze-dried
Storage conditions
2°C to 8°C

Characteristics
Comments
Cell wall and DNA analyses
Handling information
Medium
BioVector Medium 1490: Modified chopped meat medium
Temperature
30°C
Atmosphere
Anaerobic
Handling procedure
Open vial according to enclosed instructions.
Under anaerobic conditions, withdraw 0.5 ml of #1490 broth from a single test tube (5 to 6 ml) with a Pasteur or 1.0 ml pipette.  Rehydrate the entire pellet.
Aseptically transfer this aliquot back into the broth tube.  Mix well.
Use several drops of the suspension to inoculate a slant and a pre-reduced #260 blood plate. An aerobic blood plate may be streaked to check for purity.
Incubate the tubes and plate under anaerobic conditions at 30°C for 72 hours. Incubate the purity plate aerobically at 30°C.


ANAEROBIC CONDITIONS:  

Anaerobic conditions for transfer may be obtained by either of the following:

Use of an anaerobic gas chamber, or  
Placement of test tubes under a gassing cannula system hooked to anaerobic gas.  

Anaerobic conditions for incubation may be obtained by any of the following:

Loose screw caps on test tubes in anaerobic chamber,                      
Loose screw caps on test tubes in an activated anaerobic gas pack jar, or  
Use of sterile butyl rubber stoppers on test tubes so that an anaerobic gas headspace is retained.  


Handling notes
Always use freshly prepared pre-reduced media or pre-reduced media that has been previously prepared but stored under anaerobic conditions.  Resazurin in the media is a color indicator for anaerobic conditions.  Observance of pink color in medium before use or during incubation shows anaerobic conditions have not been met and oxidation has occurred.  Medium should be discarded.

Supplier来源:BioVector NTCC Inc.
TEL电话:400-800-2947
Website网址: http://www.biovector.net