Human AXL knockout HeLa cell line Axl基因敲除HeLa细胞株 BioVector NTCC质粒载体菌种细胞基因保藏中心

Human AXL knockout HeLa cell line Axl基因敲除HeLa细胞株

Parental Cell Line
HeLa
Organism
Human
Mutation description
Knockout achieved by using CRISPR/Cas9, 1 bp deletion in exon 4 and 4 bp deletion in exon 4
Passage number
<20<>Knockout validation
Sanger Sequencing, Western Blot (WB)
Tested applications
Suitable for: WBmore details
Biosafety level
2
General notes
Recommended control: Human wild-type HeLa cell line. Please note a wild-type cell line is not automatically included with a knockout cell line order, if required please add recommended wild-type cell line at no additional cost using the code WILDTYPE-TMTK1.

Cryopreservation cell medium: Cell Freezing Medium-DMSO Serum free media, contains 8.7% DMSO in MEM supplemented with methyl cellulose.

Culture medium: DMEM (High Glucose) + 10% FBS

Initial handling guidelines: Upon arrival, the vial should be stored in liquid nitrogen vapor phase and not at -80°C. Storage at -80°C may result in loss of viability.

1. Thaw the vial in 37°C water bath for approximately 1-2 minutes.
2. Transfer the cell suspension (0.8 mL) to a 15 mL/50 mL conical sterile polypropylene centrifuge tube containing 8.4 mL pre-warmed culture medium, wash vial with an additional 0.8 mL culture medium (total volume 10 mL) to collect remaining cells, and centrifuge at 201 x g (rcf) for 5 minutes at room temperature. 10 mL represents minimum recommended dilution. 20 mL represents maximum recommended dilution.
3. Resuspend the cell pellet in 5 mL pre-warmed culture medium and count using a haemocytometer or alternative cell counting method. Based on cell count, seed cells in an appropriate cell culture flask at a density of 2x104 cells/cm2. Seeding density is given as a guide only and should be scaled to align with individual lab schedules.
4. Incubate the culture at 37°C incubator with 5% CO2. Cultures should be monitored daily.

Subculture guidelines:

All seeding densities should be based on cell counts gained by established methods.
A guide seeding density of 2x104 cells/cm2 is recommended.
A partial media change 24 hours prior to subculture may be helpful to encourage growth, if required.

Number of cells
1 x 106 cells/vial, 1 mL
Viability
~80%
Adherent /Suspension
Adherent
Tissue
Cervix
Cell type
epithelial
Disease
Adenocarcinoma
Gender
Female
STR Analysis
Amelogenin X D5S818: 11, 12 D13S317: 12, 13.3 D7S820: 8, 12 D16S539: 9, 10 vWA: 16, 18 TH01: 7 TPOX: 8,12 CSF1PO: 9, 10
Antibiotic resistance
Puromycin 1.00µg/ml
Mycoplasma free
Yes
Storage instructions
Shipped on Dry Ice. Store in liquid nitrogen.
Storage buffer
Constituents: 8.7% Dimethylsulfoxide, 2% Cellulose, methyl ether
Research areas
Cell Biology Cell Cycle Kinases/Phosphatases OtherTags & Cell Markers Cell Type Markers Tumor AssociatedSignal Transduction Protein Phosphorylation Tyrosine Kinases Receptor Tyrosine KinasesCancer Cell cycle Kinases/phosphatases OtherCancer Signal transduction OtherCancer Oncoproteins/suppressors Oncoproteins Signal transducersNeuroscience Processes

Function
May function as a signal transducer between specific cell types of mesodermal origin. In case of filovirus infection, seems to function as a cell entry factor.
Tissue specificity
Highly expressed in metastatic colon tumors. Expressed in primary colon tumors. Weakly expressed in normal colon tissue.
Sequence similarities
Belongs to the protein kinase superfamily. Tyr protein kinase family. AXL/UFO subfamily.
Contains 2 fibronectin type-III domains.
Contains 2 Ig-like C2-type (immunoglobulin-like) domains.
Contains 1 protein kinase domain.
Cellular localization
Membrane.
Target information above from: UniProt accession P30530
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010) .


Cells should be passaged when they have achieved 80-90% confluence.

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